Department of Medical Physics, Faculty of Medicine, Isfahan University of Medical Science, Isfahan, Iran.
Faculty of Medicine, Semnan University of Medical Science, Semnan, Iran.
Can Respir J. 2020 Nov 28;2020:4648307. doi: 10.1155/2020/4648307. eCollection 2020.
The globally inimitable and unremitting outbreak of COVID-19 infection confirmed the emergency need for critical detection of human coronavirus infections. Laboratory diagnostic tests and imaging modalities are two test groups used for the detection of COVID-19. Nowadays, real-time polymerase chain reaction (RT-PCR) and computed tomography (CT) have been frequently utilized in the clinic. Some limitations that confront with these tests are false-negative results, tests redone for follow-up procedure, high cost, and unable to do for all patients. To overcome these limitations, modified and alternative tests must be considered. Among these tests, RdRp/Hel RT-PCR assay had the lowest diagnostic limitation and highest sensitivity and specificity for the detection of SARS-CoV-2 RNA in both respiratory tract and nonrespiratory tract clinical specimens. On the other hand, lung ultrasound (LUS) and magnetic resonance imaging (MRI) are CT-alternative imaging modalities for the management, screening, and follow-up of COVID-19 patients.
全球范围内独特且持续不断的 COVID-19 感染爆发证实了对人类冠状病毒感染进行危急检测的紧急需求。实验室诊断检测和影像学方式是用于检测 COVID-19 的两种检测方法。目前，实时聚合酶链反应（RT-PCR）和计算机断层扫描（CT）已在临床上频繁使用。这些检测方法存在一些局限性，例如假阴性结果、为后续程序重新进行检测、成本高以及无法对所有患者进行检测。为了克服这些局限性，必须考虑改进和替代检测方法。在这些检测方法中，RdRp/Hel RT-PCR 检测法在检测呼吸道和非呼吸道临床标本中的 SARS-CoV-2 RNA 时，具有最低的诊断局限性、最高的灵敏度和特异性。另一方面，肺部超声（LUS）和磁共振成像（MRI）是用于 COVID-19 患者管理、筛查和随访的 CT 替代影像学方法。
