Wilkie Jessica, Cameron Timothy C, Beddoe Travis
Centre for Livestock Interactions with Pathogens (CLiP), La Trobe University, Bundoora, VIC, Australia.
Department of Animal, Plant and Soil Science and Centre for AgriBioscience (AgriBio), La Trobe University, Bundoora, VIC, Australia.
PeerJ. 2020 Dec 7;8:e10503. doi: 10.7717/peerj.10503. eCollection 2020.
is the causative agent of fasciolosis, an important disease of humans and livestock around the world. There is an urgent requirement for novel treatments for due to increasing reports of drug resistance appearing around the world. The outer body covering of is referred to as the tegument membrane which is of crucial importance for the modulation of the host response and parasite survival; therefore, tegument proteins may represent novel drug or vaccine targets. Previous studies have identified a profilin-like protein in the tegument of . Profilin is a regulatory component of the actin cytoskeleton in all eukaryotic cells, and in some protozoan parasites, profilin has been shown to drive a potent IL-12 response. This study characterized the identified profilin form (termed Profilin) for the first time. Recombinant expression of Profilin resulted in a protein approximately 14 kDa in size which was determined to be dimeric like other profilins isolated from a range of eukaryotic organisms. Profilin was shown to bind poly-L-proline (pLp) and sequester actin monomers which is characteristic of the profilin family; however, there was no binding of Profilin to phosphatidylinositol lipids. Despite Profilin being a component of the tegument, it was shown not to generate an immune response in experimentally infected sheep or cattle.
是肝片吸虫病的病原体，肝片吸虫病是一种在世界各地对人类和家畜都很重要的疾病。由于世界各地出现耐药性的报道日益增多，迫切需要针对它的新治疗方法。的体外覆盖物被称为皮层膜，这对于调节宿主反应和寄生虫存活至关重要；因此，皮层蛋白可能代表新的药物或疫苗靶点。先前的研究在的皮层中鉴定出一种类肌动蛋白结合蛋白。肌动蛋白结合蛋白是所有真核细胞中肌动蛋白细胞骨架的调节成分，并且在一些原生动物寄生虫中，肌动蛋白结合蛋白已被证明能引发强烈的IL-12反应。本研究首次对鉴定出的来自的肌动蛋白结合蛋白（称为肌动蛋白结合蛋白）进行了表征。肌动蛋白结合蛋白的重组表达产生了一种大小约为14 kDa的蛋白质，该蛋白质被确定为二聚体，与从一系列真核生物中分离出的其他肌动蛋白结合蛋白一样。肌动蛋白结合蛋白被证明能结合聚-L-脯氨酸（pLp）并隔离肌动蛋白单体，这是肌动蛋白结合蛋白家族的特征；然而，肌动蛋白结合蛋白不与磷脂酰肌醇脂质结合。尽管肌动蛋白结合蛋白是皮层的一个组成部分，但在实验感染的绵羊或牛中，它并未引发免疫反应。
