Akbiyik A, Sari D, Ercan U K, Uyanikgil Y, Taşli H, Tomruk C, Usta Y H
Faculty of Health Sciences, Izmir Katip Celebi University, Çiğli İzmir, Turkey.
Department of Fundamentals of Nursing, Faculty of Nursing, Ege University, Izmir, Turkey.
J Appl Microbiol. 2021 Aug;131(2):973-987. doi: 10.1111/jam.14980. Epub 2021 Jan 11.
To evaluate the antimicrobial efficacy and wound healing effect of atmospheric pressure cold plasma (APCP) on an infected pressure ulcer (IPUs) model that was created on rats.
A total of 18 rats was divided into APCP, silver sulfadiazine (AgS) and control groups to have six rats in each group. A third-grade pressure ulcer model was developed on the back of each of the rats, and pressure ulcers were infected by inoculation of multidrug resistance (MDR) Pseudomonas aeruginosa. A portable dielectric barrier discharge device was used to generate cold air plasma. APCP, AgS and saline treatments were carried out once a day for 14 days. The effectiveness of the treatment was evaluated on days 5, 10 and 15. Surface area, depth, pressure ulcer healing scale (PUSH) and microbiological examination were used for evaluation.
The results of this study showed that APCP was superior over AgS application and irrigation with saline by means of the reduction in surface area and depth of ulcers. Furthermore, PUSH score in plasma group was lower than other groups and histopathological examination showed a higher epithelization in APCP group. The average reductions of MDR P. aeruginosa for APCP, AgS and control groups were determined as 5·64 ± 1·87, 1·91 ± 0·90 and 1·22 ± 0·88 log10 CFU per gram tissue, respectively.
Atmospheric pressure cold plasma healed IPUs better than AgS.
Portable cold plasma devices could be a potential novel treatment modality for the patients who have IPUs.
评估常压冷等离子体（APCP）对在大鼠身上构建的感染性压疮（IPU）模型的抗菌效果和伤口愈合作用。
将18只大鼠分为APCP组、磺胺嘧啶银（AgS）组和对照组，每组6只。在每只大鼠背部建立Ⅲ级压疮模型，并通过接种多重耐药（MDR）铜绿假单胞菌使压疮感染。使用便携式介质阻挡放电装置产生冷空气等离子体。APCP、AgS和生理盐水处理每天进行1次，共14天。在第5、10和15天评估治疗效果。使用表面积、深度、压疮愈合量表（PUSH）和微生物学检查进行评估。
本研究结果表明，通过减少溃疡的表面积和深度，APCP在应用上优于AgS和生理盐水冲洗。此外，等离子体组的PUSH评分低于其他组，组织病理学检查显示APCP组上皮化程度更高。APCP组、AgS组和对照组的MDR铜绿假单胞菌平均减少量分别确定为每克组织5·64±1·87、1·91±0·90和1·22±0·88 log10 CFU。
常压冷等离子体治疗IPU的效果优于AgS。
便携式冷等离子体装置可能是治疗IPU患者的一种潜在新型治疗方式。
