Laser applications in medical sciences research center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Laser - Plasma Research Institute, Shahid Beheshti University, G.C., P.O. Box, 19839-6941, Tehran, Iran.
Sci Rep. 2018 Apr 4;8(1):5621. doi: 10.1038/s41598-018-24049-z.
According to high incidence and prevalence of pressure ulcers worldwide, the purpose of this study is using of non-thermal atmospheric plasma as a novel therapy for pressure ulcers. Cold plasma was produced by applying a high-voltage (5 kV) and high-frequency (25 kHz), to helium gas. Under general anesthesia and sterile conditions, two circular magnets were used to create pressure ulcers on the dorsal skin of adult rats. The wounds were divided randomly into control and plasma-treated groups. Animals in the plasma-treated group received plasma radiation for 5 days, each day 3 times and every time 60 s. Mechanical assays were performed to determine plasma effects on the mechanical strength of the repaired tissue. The results showed that mechanical strength of repaired wound in the plasma-treated group was significantly higher than that in the control group (p < 0.05). In addition, evidence from histological studies indicates a significantly accelerated wound re-epithelialization in comparison with the control group; angiogenesis and fibrosis (collagen synthesis) were also significantly increased and the inflammation phase of wound healing was shorter in the plasma-treated group. The plasma treatment also resulted in significant wound contraction and acceleration of wound healing. The findings of present study indicate the effects of cold plasma on pressure ulcer treatment.
根据全球范围内压疮的高发病率和患病率，本研究旨在将非热常压等离子体作为一种治疗压疮的新方法。冷等离子体通过施加 5 kV 的高压和 25 kHz 的高频电流到氦气中产生。在全身麻醉和无菌条件下，使用两个圆形磁铁在成年大鼠的背部皮肤造成压疮。将伤口随机分为对照组和等离子体处理组。等离子体处理组的动物接受等离子体辐射 5 天，每天 3 次，每次 60 秒。进行力学测定以确定等离子体对修复组织机械强度的影响。结果表明，等离子体处理组修复伤口的机械强度明显高于对照组（p<0.05）。此外，组织学研究的证据表明，与对照组相比，伤口再上皮化明显加快；血管生成和纤维化（胶原蛋白合成）也明显增加，等离子体处理组的炎症期愈合缩短。等离子体处理还导致伤口明显收缩和愈合加速。本研究的结果表明，冷等离子体对压疮治疗有一定的影响。
