Department of Biotechnology, National Institute of Technology, Mahatma Gandhi Rd, A-zone, Durgapur, West Bengal-713209, India.
Department of Life Sciences, Presidency University, 86/1 College street, Kolkata, West Bengal-700073, India.
FEMS Microbiol Lett. 2021 Jan 26;368(1). doi: 10.1093/femsle/fnaa216.
Rice blast caused by Magnaporthe oryzae continues to be a major constraint in rice production worldwide. Rice is one of the staple crops in India and rice blast causes huge economic losses. Interestingly, the Indian subcontinent is the centre for origin and diversity of rice as well as the Magnaporthe species complex. Secondary metabolites are known to play important role in pathogenesis and M. oryzae has high potential of genes involved in secondary metabolism but, unfortunately most of them remain uncharacterized. In the present study, we analysed the draft genome assemblies of M. oryzae strains isolated from different parts of India, for putative secondary metabolite key gene (SMKG) clusters encoding polyketide synthases, non-ribosomal peptide synthetases, diterpene cyclases and dimethylallyl tryptophan synthase. Based on the complete genome sequence of 70-15 strain and its previous reports of identified SMKGs, we have identified the key genes for the interrogated strains. Expression analysis of these genes amongst different strains indicates how they have evolved depending on the host and environmental conditions. To our knowledge, this study is first of its kind where the secondary metabolism genes and their role in functional adaptation were studied across several strains of M. oryzae.
稻瘟病由稻瘟病菌引起，仍然是全球水稻生产的主要制约因素。水稻是印度的主要粮食作物之一，稻瘟病造成了巨大的经济损失。有趣的是，印度次大陆是水稻和稻瘟病菌种复合体的起源和多样性中心。次生代谢物在发病机制中起着重要作用，稻瘟病菌具有参与次生代谢的高潜力基因，但不幸的是，其中大多数仍未被描述。在本研究中，我们分析了从印度不同地区分离的稻瘟病菌株的基因组草图，以确定编码聚酮合酶、非核糖体肽合酶、二萜环化酶和二甲基烯丙基色氨酸合酶的假定次生代谢物关键基因（SMKG）簇。基于 70-15 菌株的完整基因组序列及其先前报道的鉴定的 SMKGs，我们确定了被研究菌株的关键基因。对这些基因在不同菌株中的表达分析表明了它们如何根据宿主和环境条件进化。据我们所知，这项研究是首次对稻瘟病菌的次生代谢基因及其在功能适应中的作用进行了多菌株研究。
