Dauda Wadzani Palnam, Morumda Daji, Abraham Peter, Adetunji Charles Oluwaseun, Ghazanfar Shakira, Glen Elkanah, Abraham Shittu Emmanuel, Peter Grace Wabba, Ogra Israel Ogwuche, Ifeanyi Ulasi Joseph, Musa Hannatu, Azameti Mawuli Kwamla, Paray Bilal Ahamad, Gulnaz Aneela
Crop Science Unit, Department of Agronomy, Federal University Gashua, Gashua P.M.B. 1005, Yobe State, Nigeria.
Department of Microbiology, Federal University Wukari, Wukari P.M.B. 1020, Taraba State, Nigeria.
J Fungi (Basel). 2022 Mar 22;8(4):324. doi: 10.3390/jof8040324.
Cytochrome P450s are a group of monooxygenase enzymes involved in primary, secondary and xenobiotic metabolisms. They have a wide application in the agriculture sector where they could serve as a target for herbicides or fungicides, while they could function in the pharmaceutical industry as drugs or drugs structures or for bioconversions. species are among the most commonly encountered fungal genera, with most of them living as saprophytes in different habitats, while others are parasites of plants and animals. This study was conducted to elucidate the diversity and abundance, evolutionary relationships and cellular localization of 372 cytochrome P450 in 13 species. The 372 CYP proteins were phylogenetically clustered into ten clades. Forty (40) clans and seventy-one (71) cyp families were identified, of which eleven (11) families were found to appear in one species each. The majority of the CYP proteins were located in the endomembrane system. Polyketide synthase (PKS) gene cluster was the predominant secondary metabolic-related gene cluster in all the studied, except in , where non-ribosomal peptide synthetase genes were dominant. This study reveals the expansion of cyps in these fungal genera, evident in the family and clan expansions, which is usually associated with the evolution of fungal characteristics, especially their lifestyle either as parasites or saprophytes, with the ability to metabolize a wide spectrum of substrates. This study can be used to understand the biology, physiology and toxigenic potentials of P450 in these fungal genera.
细胞色素P450是一组参与初级、次级和外源性代谢的单加氧酶。它们在农业领域有广泛应用，可作为除草剂或杀菌剂的作用靶点，同时在制药行业中可作为药物、药物结构或用于生物转化。某些物种是最常见的真菌属，其中大多数在不同生境中以腐生菌形式生存，而其他一些则是动植物的寄生虫。本研究旨在阐明13个物种中372种细胞色素P450的多样性和丰度、进化关系及细胞定位。这372种CYP蛋白在系统发育上聚类为十个分支。鉴定出40个族和71个CYP家族，其中有11个家族分别只出现在一个物种中。大多数CYP蛋白位于内膜系统中。聚酮合酶（PKS）基因簇是所有研究物种中主要的次级代谢相关基因簇，但在[具体物种名称缺失]中除外，在该物种中非核糖体肽合成酶基因占主导地位。本研究揭示了这些真菌属中CYP的扩增，这在家族和族的扩增中很明显，通常与真菌特性的进化相关，特别是它们作为寄生虫或腐生菌的生活方式以及代谢多种底物的能力。本研究可用于了解这些真菌属中P450的生物学、生理学和产毒潜力。
