College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica, Guangxi University of Chinese Medicine, Nanning, China.
Pharm Biol. 2020 Dec;58(1):1294-1301. doi: 10.1080/13880209.2020.1859553.
Although the roots and stems of (Lem.) A. C. Smith. [Schisandraceae] are herbs and traditional foods in Li nationality, its toxicity remains unclear.
To study developmental toxicity of consumption and explain underlying mechanisms.
Zebrafish were applied to assess LC values of hydroethanol extract (KCH) and water extract (KCW) of . In further study, three concentrations groups of KCH (3.75, 7.5 and 15 μg/mL for embryo, 7.5, 15 and 30 μg/mL for larvae) and control group ( = 30) were administered. At specific stages of zebrafish development, spontaneous movement, hatching rate, etc., were measured. Gene expressions related to developmental toxicity were examined.
The LC value of KCH (24 or 45 μg/mL) was lower than KCW (1447 or 2011 μg/mL) in embryos or larvae. The inhibited spontaneous movement (20%), hatching rate (20%), body length (12%) and eye area (30%) were observed after KCH treatment. Moreover, the decreased liver areas (25%) and fluorescence intensity (33%), increased ALT (37%) and AST levels (42%) were found in larvae treated with KCH (30 μg/mL). The increased ROS (89%), MDA concentrations (30%), apoptosis generation (62%) and decreased T-SOD activity (16%) were also observed. The represented genes of developmental hepatotoxicity, oxidative stress and apoptosis in zebrafish were activated after KCH (15 or 30 μg/mL) treatment.
These results demonstrate that KCH has developmental toxicity on zebrafish. Our study provides a scientific basis for further research on the toxicity of .
（五味子 A. C. 史密斯。[五味子科]）的根和茎是黎族的草药和传统食物，但毒性尚不清楚。
研究五味子食用的发育毒性，并解释其潜在机制。
利用斑马鱼评估水醇提取物（KCH）和水提物（KCW）的 LC 值。在进一步的研究中，将 KCH（胚胎为 3.75、7.5 和 15μg/mL，幼虫为 7.5、15 和 30μg/mL）和对照组（n=30）分为三个浓度组。在斑马鱼发育的特定阶段，测量自发运动、孵化率等。检测与发育毒性相关的基因表达。
KCH（胚胎为 24 或 45μg/mL，幼虫为 1447 或 2011μg/mL）的 LC 值低于 KCW。KCH 处理后观察到自发运动（20%）、孵化率（20%）、体长（12%）和眼面积（30%）抑制。此外，KCH（30μg/mL）处理的幼虫肝面积（25%）和荧光强度（33%）降低，ALT（37%）和 AST 水平（42%）升高。还观察到 ROS（89%）、MDA 浓度（30%）增加、凋亡生成（62%）和 T-SOD 活性（16%）降低。KCH（15 或 30μg/mL）处理后，斑马鱼发育性肝毒性、氧化应激和凋亡的代表性基因被激活。
这些结果表明 KCH 对斑马鱼具有发育毒性。本研究为进一步研究五味子的毒性提供了科学依据。
