Department of Food Science and Technology, Chonnam National University, Gwangju, 61186, Republic of Korea.
Food Funct. 2019 Aug 1;10(8):4636-4648. doi: 10.1039/c9fo00982e. Epub 2019 Jul 10.
This study aimed to evaluate the potentially beneficial effects of alginate oligosaccharide (AOS) on the modulation of immuno-metabolic pathways in high-fat-diet (HFD)-induced obese zebrafish and the underlying mechanism. AOS showed a marked anti-obesity effect in that it reduced body weight, BMI, and the blood glucose level. To understand the mechanisms of action of AOS, comparative proteomics was performed through UPLC-HDMS analysis between HFD vs. normal diet (NFD) and HFD + AOS vs. HFD. Among 146 proteins differentially modulated by AOS in HFD-induced obesity zebrafish, STOML2 (Stomatin-like protein 2) was selected as a specific biomarker. AOS suppressed obesity and pathophysiological disorders in HFD-fed zebrafish by modulating lipid metabolism, suppressing inflammation, downregulating apoptosis-related genes, and improving immune function by inhibiting STOML2. Our results suggest that STOML2 can serve as a platform for further studies to discover novel treatments for metabolic disorders. AOS might be useful as a dietary health supplement, especially for reducing obesity.
本研究旨在评估海藻酸钠寡糖（AOS）对高脂肪饮食（HFD）诱导肥胖斑马鱼免疫代谢途径的调节作用及其潜在的有益影响和作用机制。AOS 表现出明显的抗肥胖作用，可降低体重、BMI 和血糖水平。为了了解 AOS 的作用机制，通过 UPLC-HDMS 分析在 HFD 与正常饮食（NFD）和 HFD+AOS 与 HFD 之间进行了比较蛋白质组学。在 AOS 调节 HFD 诱导肥胖斑马鱼的 146 种差异表达蛋白中，选择 STOML2（Stomatin-like protein 2）作为特定的生物标志物。AOS 通过调节脂质代谢、抑制炎症、下调与凋亡相关的基因、抑制 STOML2 来改善免疫功能，从而抑制 HFD 喂养的斑马鱼肥胖和病理生理紊乱。我们的研究结果表明，STOML2 可以作为进一步研究代谢紊乱新疗法的平台。AOS 可能作为膳食保健品很有用，尤其是用于减肥。
