在活细胞中通过自组装纳米纤维探针实时监测半胱天冬酶-3/8 的活性。
Real-time monitoring of caspase-3/8 activity by self-assembling nanofiber probes in living cells.
机构信息
State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, NanKai University, Tianjin 300350, China.
出版信息
Chem Commun (Camb). 2021 Jan 18;57(6):797-800. doi: 10.1039/d0cc07821b. Epub 2020 Dec 23.
PMID:33355564
Abstract
Caspase-3/8 are key members of the cysteine-aspartyl protease family with pivotal roles in apoptosis. We have designed and synthesized self-assembling probes, Nap-GFFpYDEVD-AFC and Nap-GFFpYIETD-AFC, with fluorescence 'turn-on' properties for real-time monitoring of Caspase-3/8 activity in living cells.
摘要
半胱氨酸天冬氨酸蛋白酶家族中的关键成员 Caspase-3/8 在细胞凋亡中起着关键作用。我们设计并合成了具有荧光“开启”特性的自组装探针 Nap-GFFpYDEVD-AFC 和 Nap-GFFpYIETD-AFC，可实时监测活细胞中 Caspase-3/8 的活性。
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