Department of Gastroenterology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China.
Department of Gastroenterology, Affiliated Hospital of Nantong University, Nantong, Jiangsu, China.
Cancer Invest. 2021 Apr;39(4):297-309. doi: 10.1080/07357907.2020.1870125. Epub 2021 Jan 8.
To investigate how EHD2 influences the development of colon cancer. Immunohistochemistry of 90 colon cancer tissue specimens were determined the expression of EHD2. The lentivirus-EHD2-transfected colon cancer cells were conducted to evaluate the biological behaviors. EHD2 was closely associated with clinic pathological parameters ( < 0.001). EHD2 upregulation was relative with a longer overall survival. The results of the univariate and multivariate analyses indicated that EHD2 could be an independent prognosis marker. EHD2 overexpression suppressed cell invasion and proliferation, but enhanced cell apoptosis and cell cycle arrest. EHD2 might represent a therapeutic target of colon cancer.IMPACT STATEMENT Membrane trafficking is crucial for cell proliferation, differentiation and apoptosis, especially tumorigenesis and development. EHD2 proteins play an important role in the regulation of membrane trafficking in endocytosis. EHD2 has been suggested to participate in the occurrence of some malignancies. EHD2 could be an independent prognosis marker in colon cancer. EHD2 overexpression suppressed cell invasion and proliferation, but enhanced cell apoptosis and cell cycle arrest . EHD2 overexpression markedly increased the expression of EMT marker E-cadherin in colon cancer. EHD2 overexpression may inhibit tumorigenesis in colon cancer through the modulation of E-cadherin, the critical marker of EMT which is closely related to invasion and distant metastasis of tumor cells.
为了研究 EHD2 如何影响结肠癌的发展。通过免疫组织化学方法检测 90 例结肠癌组织标本中 EHD2 的表达情况。转染 EHD2 的慢病毒结肠癌细胞系被用来评估其生物学行为。EHD2 与临床病理参数密切相关（<0.001）。EHD2 上调与总生存期延长相关。单因素和多因素分析结果表明，EHD2 可以作为独立的预后标志物。EHD2 过表达抑制细胞侵袭和增殖，但增强细胞凋亡和细胞周期阻滞。EHD2 可能代表结肠癌的治疗靶点。
膜转运对于细胞增殖、分化和凋亡，特别是肿瘤的发生和发展至关重要。EHD2 蛋白在细胞内吞作用中对膜转运的调节中发挥重要作用。EHD2 被认为参与了一些恶性肿瘤的发生。EHD2 可以作为结肠癌的独立预后标志物。EHD2 过表达抑制细胞侵袭和增殖，但增强细胞凋亡和细胞周期阻滞。EHD2 过表达显著增加了结肠癌中 EMT 标志物 E-钙黏蛋白的表达。EHD2 过表达可能通过调节 EMT 的关键标志物 E-钙黏蛋白来抑制结肠癌的肿瘤发生，E-钙黏蛋白与肿瘤细胞的侵袭和远处转移密切相关。
