Department of Orthopedics, The Affiliated Changzhou No.2 People's Hospital of Nanjing Medical University, Changzhou China.
Immunol Invest. 2022 Apr;51(3):660-669. doi: 10.1080/08820139.2020.1864397. Epub 2020 Dec 28.
The purposes of this study were to explore whether the Toll-like receptor 4 () gene rs7873784 G/C polymorphism was related to the risk of rheumatoid arthritis (RA) and to the clinical features of the disease in Chinese subjects.
We examined the rs7873784 G/C polymorphism in 805 Chinese RA patients and 1095 healthy controls. Genotype was determined with a custom-by-design 48-Plex single nucleotide polymorphism scan™ Kit. Blood plasma levels of TLR4 in 170 RA patients and 170 matched controls were measured by ELISA.
The gene rs7873784 G/C polymorphism was related to a reduced risk for RA. By stratified analysis, we found a dramatically reduced risk for RA in patients who were female, CRP-positive, RF-positive, DAS28 ≥ 3.20, or ESR ≥ 25. Compared with the controls, the average level of TLR4 protein in plasma of RA patients was increased. In addition, RA patients exhibited higher levels of mRNA than controls ( < .05).
These results demonstrate the rs7873784 G/C polymorphism to relate to a decreased risk for RA in a Chinese population.
本研究旨在探讨 Toll 样受体 4（）基因 rs7873784 G/C 多态性是否与中国人群类风湿关节炎（RA）的发病风险及疾病临床特征相关。
我们检测了 805 例中国 RA 患者和 1095 例健康对照者的 rs7873784 G/C 多态性。采用定制设计的 48-Plex 单核苷酸多态性扫描试剂盒确定基因型。通过 ELISA 法检测 170 例 RA 患者和 170 例匹配对照者的血浆 TLR4 水平。
基因 rs7873784 G/C 多态性与 RA 的发病风险降低相关。通过分层分析，我们发现女性、CRP 阳性、RF 阳性、DAS28≥3.20 或 ESR≥25 的患者发生 RA 的风险显著降低。与对照组相比，RA 患者血浆中 TLR4 蛋白的平均水平升高。此外，与对照组相比，RA 患者的 mRNA 水平更高（<0.05）。
这些结果表明，在中国人群中，rs7873784 G/C 多态性与 RA 的发病风险降低相关。
