Lamers W H, Janzen J W, Moorman A F, Charles R, Knecht E, Martínez-Ramón A, Hernández-Yago J, Grisolía S
Department of Anatomy and Embryology, University of Amsterdam AMC, The Netherlands.
J Histochem Cytochem. 1988 Jan;36(1):41-7. doi: 10.1177/36.1.3335769.
In adult rat liver, glutamate dehydrogenase is present in high concentrations around the terminal portal (zone 1) and hepatic (zone 3) veins, whereas its concentration is low in the intermediate zone. Although the size and staining intensity of the periportal glutamate dehydrogenase-positive compartment are less than those of the pericentral compartment, it can expand under appropriate endocrine conditions, leading to a homogeneous distribution. At birth, glutamate dehydrogenase is also homogeneously distributed. Glutamate dehydrogenase disappears from the periportal compartment during the first postnatal week and reappears in that compartment after weaning. These observations indicate an independent regulation of glutamate dehydrogenase levels in the periportal and pericentral zone. The size of the periportal glutamate dehydrogenase-containing zone is appreciably smaller than that of carbamoylphosphate synthetase, whereas the pericentral glutamate dehydrogenase-containing zone is appreciably larger than that of glutamine synthetase. The heterogeneous distribution of glutamate dehydrogenase suggests the possibility that, under normal conditions, deamination of glutamate prevails in the periportal compartment and amination of glutamate in the pericentral compartment.
在成年大鼠肝脏中，谷氨酸脱氢酶在终末门静脉（1区）和肝静脉（3区）周围浓度较高，而在中间区域浓度较低。尽管门静脉周围谷氨酸脱氢酶阳性区的大小和染色强度小于中央周围区，但在适当的内分泌条件下它会扩大，导致均匀分布。出生时，谷氨酸脱氢酶也是均匀分布的。出生后第一周，门静脉周围区的谷氨酸脱氢酶消失，断奶后又重新出现。这些观察结果表明门静脉周围区和中央周围区的谷氨酸脱氢酶水平存在独立调节。门静脉周围含谷氨酸脱氢酶区域的大小明显小于氨基甲酰磷酸合成酶区域，而中央周围含谷氨酸脱氢酶区域明显大于谷氨酰胺合成酶区域。谷氨酸脱氢酶的异质分布表明，在正常情况下，门静脉周围区以谷氨酸脱氨为主，而中央周围区以谷氨酸胺化为主。
