Department of Animal Biotechnology and Genetics, Faculty of Animal Breeding and Biology, University of Science and Technology in Bydgoszcz, 85-084 Bydgoszcz, Poland.
Department of Animal Biotechnology and Genetics, Faculty of Animal Breeding and Biology, University of Science and Technology in Bydgoszcz, 85-084 Bydgoszcz, Poland.
Poult Sci. 2021 Jan;100(1):319-324. doi: 10.1016/j.psj.2020.08.016. Epub 2020 Aug 26.
The Green-legged Partridgelike fowl is an old Polish indigenous breed of chicken. Primordial germ cells (PGCs) are one of the best sources of precursor cells that can be used for the conservation and proliferation of the endangered breeds of bird. Initially, the chicken PGCs colonize at the anterior extraembryonic region called "germinal crescent," and after the establishment of blood vascular circulation, they temporally circulate via the embryonic blood vascular system along with embryonic blood cells. They further colonize at the microcapillary networks of both right and left future gonadal regions. Subsequently, they migrate interstitially to reach gonadal anlages, where they begin to differentiate and eventually develop into the future ova or sperm. The basic knowledge regarding the concentration and the total number of circulating PGCs (cPGCs) throughout their circulating phase in the early embryonic stages is crucial for providing an insight into the mechanisms by which they circulate and colonize at the capillary networks of left and right future gonadal regions in each developmental stage. The present study aims to determine the most efficient developmental stage that is suitable to collect cPGCs. The concentration of cPGCs was directly measured, and total volume of embryonic blood was calculated based on the concentration of PKH26-stained embryonic blood cells which were injected 10 min before the blood sampling process in the same embryo during each stage of embryonic development from stage 13 Hamburger and Hamilton (HH; Hamburger and Hamilton, 1951) to 16 HH. Analysis of whole embryonic bloodstream revealed that at stage 14 HH of embryonic development, peak total number of cPGCs (386.3 cells/μL) and peak concentration of cPGCs (18.6 cells/μL) were observed. Later, there was a decrease in concentration, suggesting that the cPGCs might be trapped gradually by the capillary networks at the future gonadal regions after stage 15 HH.
绿脚雉鸡是一种古老的波兰本土鸡品种。原始生殖细胞（PGC）是可用于保护和繁殖濒危鸟类的前体细胞的最佳来源之一。最初，鸡 PGC 在前胚胎外区域“生殖新月”中定殖，在建立血管循环后，它们与胚胎血细胞一起通过胚胎血管系统暂时循环。然后，它们在左右未来性腺区域的微毛细血管网络中定植。随后，它们间质迁移到达性腺原基，在那里它们开始分化，并最终发育成未来的卵子或精子。了解它们在早期胚胎阶段循环过程中的浓度和循环 PGC（cPGC）总数，对于深入了解它们在每个发育阶段如何在左右未来性腺区域的毛细血管网络中循环和定植的机制至关重要。本研究旨在确定最适合收集 cPGC 的最有效发育阶段。直接测量 cPGC 的浓度，并根据在胚胎发育的第 13 期 Hamburger 和 Hamilton（HH；Hamburger 和 Hamilton，1951）到第 16 期 HH 期间，同一胚胎在采血前 10 分钟注射的 PKH26 染色胚胎血细胞的浓度，计算胚胎血液的总体积。对整个胚胎血液流的分析表明，在胚胎发育的第 14 期 HH 时，观察到 cPGC 的总数量（386.3 个/μL）和浓度峰值（18.6 个/μL）。之后，浓度下降，这表明 cPGC 可能在第 15 期 HH 之后逐渐被未来性腺区域的毛细血管网络捕获。
