Tajima A, Hayashi H, Kamizumi A, Ogura J, Kuwana T, Chikamune T
Institute of Agriculture and Forestry, University of Tsukuba, Ibaraki, Japan.
J Exp Zool. 1999 Dec 1;284(7):759-64. doi: 10.1002/(sici)1097-010x(19991201)284:7<759::aid-jez5>3.0.co;2-6.
Experiments were conducted to elucidate the factor that influences the concentration of circulating primordial germ cells (cPGCs) in two-day old chick embryos. The concentration of cPGCs was observed to be highest at stage 14 (66.9 +/- 23.2 microliters) and decreased thereafter. However, considerable egg to egg variations in cPGC concentration, especially at stages 13, 14, 15, and 16 were observed. After conducting experiments to elucidate the source of egg to egg variation in cPGC concentration among embryos, it was revealed that there are hens that lay eggs which contain either constantly high (more than 80 PGCs/microliter) or constantly low (less than 30 PGCs/microliter) concentration of cPGCs. The results obtained from the present experiments showed that one of the major source of egg to egg variation in the concentration of cPGCs was due to the individual differences among females that produced the eggs.
开展实验以阐明影响两日龄鸡胚中循环原始生殖细胞（cPGCs）浓度的因素。观察到cPGCs浓度在第14阶段最高（66.9±23.2微升），此后下降。然而，观察到cPGC浓度在蛋与蛋之间存在相当大的差异，尤其是在第13、14、15和16阶段。在进行实验以阐明胚胎间cPGC浓度蛋与蛋差异的来源后，发现有些母鸡产的蛋中cPGCs浓度持续较高（超过80个PGCs/微升）或持续较低（低于30个PGCs/微升）。本实验获得的结果表明，cPGCs浓度蛋与蛋差异的主要来源之一是产蛋母鸡之间的个体差异。
