Department of Oral & Cranio-maxillofacial Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200000, China; National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai 200000, China.
National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai 200000, China; Department of Oral Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200000, China.
Chin J Nat Med. 2020 Dec;18(12):922-933. doi: 10.1016/S1875-5364(20)60036-X.
Due to the poor repair ability of cartilage tissue, regenerative medicine still faces great challenges in the repair of large articular cartilage defects. Quercetin is widely applied as a traditional Chinese medicine in tissue regeneration including liver, bone and skin tissues. However, the evidence for its effects and internal mechanisms for cartilage regeneration are limited. In the present study, the effects of quercetin on chondrocyte function were systematically evaluated by CCK8 assay, PCR assay, cartilaginous matrix staining assays, immunofluorescence assay, and western blotting. The results showed that quercetin significantly up-regulated the expression of chondrogenesis genes and stimulated the secretion of GAG (glycosaminoglycan) through activating the ERK, P38 and AKT signalling pathways in a dose-dependent manner. Furthermore, in vivo experiments revealed that quercetin-loaded silk protein scaffolds dramatically stimulated the formation of new cartilage-like tissue with higher histological scores in rat femoral cartilage defects. These data suggest that quercetin can effectively stimulate chondrogenesis in vitro and in vivo, demonstrating the potential application of quercetin in the regeneration of cartilage defects.
由于软骨组织的修复能力较差，再生医学在修复大的关节软骨缺损方面仍然面临巨大挑战。槲皮素作为一种传统中药，广泛应用于肝、骨和皮肤等组织的再生。然而，其在软骨再生方面的作用和内在机制的证据有限。在本研究中，通过 CCK8 检测、PCR 检测、软骨基质染色检测、免疫荧光检测和 Western blot 检测，系统评估了槲皮素对软骨细胞功能的影响。结果表明，槲皮素通过激活 ERK、P38 和 AKT 信号通路，呈剂量依赖性地上调软骨生成基因的表达，并刺激 GAG（糖胺聚糖）的分泌。此外，体内实验表明，负载槲皮素的丝蛋白支架可显著刺激大鼠股骨软骨缺损处新的类软骨组织的形成，具有更高的组织学评分。这些数据表明，槲皮素可以有效地在体外和体内刺激软骨生成，表明槲皮素在软骨缺损再生方面具有潜在的应用。
