Hirai K, De Weck A L, Stadler B M
Institute of Clinical Immunology, University of Bern, Inselspital, Switzerland.
J Immunol. 1988 Jan 1;140(1):221-7.
Biologic and biochemical properties of a human basophil-like cell promoting activity (BaPA), which induces growth of metachromatically staining cells from normal bone marrow cells in a liquid culture system have been examined. In order to study this T cell factor, an assay was developed based on the intracellular histamine content of the cultured human bone marrow cells. Many lymphokines, including granulocyte-macrophage colony-stimulating factor, granulocyte colony-stimulating factor, interleukin 1 alpha and 1 beta, interleukin 2, and interferon-alpha and gamma, did not exhibit any significant activity in the assay. By employing this assay, BaPA was purified approximately 500-fold from lectin-stimulated spleen cell-conditioned medium. BaPA has a molecular weight of 23,000 on high performance liquid chromatography gel filtration and displays isoelectric points between 5.8 and 7.3. It is heat stable up to 80 degrees C for 30 min and resistant to 6 M guanidine hydrochloride, whereas it is rather sensitive to sulfhydryl reagents. BaPA has no stimulating activity on mouse bone marrow cells.
已对一种人类嗜碱性粒细胞样细胞促进活性（BaPA）的生物学和生化特性进行了研究，该活性可在液体培养系统中诱导正常骨髓细胞产生异染性染色细胞的生长。为了研究这种T细胞因子，基于培养的人类骨髓细胞的细胞内组胺含量开发了一种检测方法。许多细胞因子，包括粒细胞-巨噬细胞集落刺激因子、粒细胞集落刺激因子、白细胞介素1α和1β、白细胞介素2以及干扰素-α和γ，在该检测中均未表现出任何显著活性。通过使用该检测方法，从凝集素刺激的脾细胞条件培养基中纯化出了约500倍的BaPA。在高效液相色谱凝胶过滤中，BaPA的分子量为23,000，等电点在5.8至7.3之间。它在80℃下30分钟内热稳定，对6M盐酸胍有抗性，而对巯基试剂相当敏感。BaPA对小鼠骨髓细胞没有刺激活性。
