Fishman P, Djaldetti M, Grossman S, Sofer Y, Sredni B
Department of Medicine B, Hasharon Hospital, Golda Medical Center, Petah-Tiqva, Israel.
Nat Immun Cell Growth Regul. 1990;9(5):334-41.
The production of a factor with interleukin-3-like activity (IL-3-LA) by cultured human peripheral-blood mononuclear cells has been studied. Culture supernatants spontaneously derived from lymphocytes or monocytes stimulated the proliferative activity of 3 IL-3-dependent cell lines. Purification of this factor by gel filtration-high-pressure liquid chromatography, sodium dodecyl sulfate-polyacrylamide gel electrophoresis and isoelectric focusing demonstrated the presence of a glycoprotein with a molecular weight of 25-30 kilodaltons and an isoelectric point of 7.6. The biochemical characteristics of the IL-3-LA derived from human monocytes and lymphocytes were very similar. The biological activity of the semipurified factor was tested on mononuclear cells from fetal cord blood. It was found that after 21 days 40% of the cells in the culture were mature basophils which released 15-18 ng histamine/10(6) cells.
已对培养的人外周血单个核细胞产生白细胞介素-3样活性因子（IL-3-LA）的情况进行了研究。淋巴细胞或单核细胞自发产生的培养上清液刺激了3种IL-3依赖细胞系的增殖活性。通过凝胶过滤-高压液相色谱、十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和等电聚焦对该因子进行纯化，结果表明存在一种糖蛋白，其分子量为25 - 30千道尔顿，等电点为7.6。源自人单核细胞和淋巴细胞的IL-3-LA的生化特性非常相似。在来自胎儿脐带血的单个核细胞上测试了半纯化因子的生物活性。结果发现，培养21天后，培养物中40%的细胞为成熟嗜碱性粒细胞，这些细胞释放15 - 18 ng组胺/10⁶个细胞。
