Universidad Autonoma de Nuevo Leon, Mechanical and Electrical Engineering School, Ave. Universidad s/n, Ciudad Universitaria, San Nicolas de los Garza, Nuevo Leon, C.P. 66455. México.
Universidad Autonoma de Nuevo Leon, Chemistry School, Ave. Universidad s/n, Ciudad Universitaria, San Nicolas de los Garza, Nuevo Leon, C.P. 66455. México.
Carbohydr Polym. 2021 Feb 15;254:117463. doi: 10.1016/j.carbpol.2020.117463. Epub 2020 Dec 1.
Better understanding through direct observation of the mechanisms involved in chemical and enzymatic hydrolysis of biomass is of great importance, to implement a substitute for the common cellulose standards. We report the hydrolysis of biomass, using exclusively the parenchyma, to isolate cellulose nanoplatelets using a less harsh pretreatment. Then, we show direct evidence of the effect of endoglucanase on the structure of cellulose nanoplatelets, finding that amorphous cellulose is exclusively digested, loosening the cellulose nanofibrils in the process. The analysis of micrographs demonstrates that when cellulose nanoplatelets are deposited on a silicon wafer, its thickness can be qualitatively measured by the interference color detected using an optical microscope. This finding facilitates further studies of mechanisms involved in lignin removal and cellulose nanofibrils production by specific enzymatic digestion.
通过直接观察生物质的化学和酶水解过程中的机制，可以更好地理解这一过程，从而实现对常见纤维素标准品的替代。我们报告了使用专门的实质组织对生物质进行水解，以采用较少苛刻的预处理方法分离纤维素纳米片。然后，我们展示了内切葡聚糖酶对纤维素纳米片结构影响的直接证据，发现无定形纤维素被完全消化，在此过程中使纤维素纳米纤维松散。通过对显微照片的分析表明，当纤维素纳米片沉积在硅片上时，其厚度可以通过光学显微镜检测到的干涉色定性测量。这一发现有助于进一步研究通过特定酶解去除木质素和生产纤维素纳米纤维的机制。
