Spector R
Department of Internal Medicine, University of Iowa College of Medicine, Iowa City.
J Neurochem. 1988 Feb;50(2):639-43. doi: 10.1111/j.1471-4159.1988.tb02958.x.
Across the cerebral capillaries, the anatomical locus of the blood-brain barrier, the unidirectional influxes of the saturated fatty acids, octanoic and myristic acids, and the unsaturated essential fatty acid, linoleic acid, were measured. Employing an in situ rat brain perfusion technique that allows control of perfusate composition and accurate measurement of perfusate-to-brain fatty acid transport, we found that both [14C]octanoic and [14C]myristic acids were transported through the blood-brain barrier in vivo, in large part, by a specific, probenecid-sensitive transport system. However, the transport of [14C]linoleic acid was not probenecid sensitive. With 0.5 microM fatty acid but no plasma proteins in the perfusate, the permeability-surface area constant was higher for myristic acid (4.8 X 10(-2) X s-1) than for octanoic and linoleic acids (1.5 and 1.2 X 10(-2) X s-1, respectively). Approximately 70, 30, and 25% of the [14C]myristic, [14C]octanoic, or [14C]linoleic acids, respectively, were extracted from the perfusate.
在脑毛细血管（血脑屏障的解剖学部位）中，对饱和脂肪酸辛酸和肉豆蔻酸以及不饱和必需脂肪酸亚油酸的单向流入进行了测量。采用一种原位大鼠脑灌注技术，该技术能够控制灌注液成分并精确测量灌注液到脑的脂肪酸转运，我们发现，[14C]辛酸和[14C]肉豆蔻酸在体内通过血脑屏障的转运，在很大程度上是通过一种特定的、对丙磺舒敏感的转运系统进行的。然而，[14C]亚油酸的转运对丙磺舒不敏感。当灌注液中脂肪酸浓度为0.5微摩尔且无血浆蛋白时，肉豆蔻酸的通透表面积常数（4.8×10⁻²×秒⁻¹）高于辛酸和亚油酸（分别为1.5×10⁻²×秒⁻¹和1.2×10⁻²×秒⁻¹）。分别从灌注液中提取了约70%、30%和25%的[14C]肉豆蔻酸、[14C]辛酸或[14C]亚油酸。
