Departamento de Tecnología de Alimentos, Universitat Politècnica de València, Spain.
Departamento de Tecnología de Alimentos, Universitat Politècnica de València, Spain.
Chem Biol Interact. 2021 Jan 25;334:109363. doi: 10.1016/j.cbi.2020.109363. Epub 2020 Dec 22.
The effect of the presence of food on the incorporation and excretion of silica particles was studied in this work using the biological model Caenorhabditis elegans and image analysis techniques. The experiment was based on two 24-hour phases: exposure and depuration. During exposure, nematodes were maintained for 24 h in liquid medium with silica particles, but some with and others without food. During depuration, nematodes were transferred to medium without particles. Nematodes were analysed by an image analysis in both phases to quantify the properties of particle distributions in nematodes' bodies with time. No differences were found in the proportion of nematodes carrying particles in the exposure phase when food was present. However in the depuration phase, lack of food generated a high proportion of particle carriers. Particle distribution properties were also similar in the exposure phase. Nevertheless, lack of food produced particle accumulation due to decelerated excretion because digestive tube relaxed under these conditions. Thus after the depuration phase, lack of food led particles to persist in digestive tubes. According to these results, intake of silica particles had no retention effects when a food flux was provided, but particles were not easily excreted when the food flux was interrupted.
本研究采用秀丽隐杆线虫生物模型和图像分析技术，研究了食物存在对硅颗粒摄入和排泄的影响。实验基于两个 24 小时的阶段：暴露和净化。在暴露阶段，线虫在含有硅颗粒的液体培养基中维持 24 小时，但有些线虫有食物，有些没有。在净化阶段，线虫被转移到没有颗粒的培养基中。在这两个阶段，通过图像分析对线虫进行分析，以随时间定量研究线虫体内颗粒分布的性质。当有食物存在时，在暴露阶段携带颗粒的线虫比例没有差异。然而，在净化阶段，缺乏食物会产生大量的颗粒载体。暴露阶段的颗粒分布特性也相似。然而，在这些条件下，由于消化管松弛，缺乏食物会导致排泄减速，从而导致颗粒积累。因此，在净化阶段之后，缺乏食物会导致颗粒在消化管中持续存在。根据这些结果，当提供食物通量时，摄入硅颗粒没有保留效应，但当食物通量中断时，颗粒不易排出。
