Alvarez Gisela S, Echazu Maria I Alvarez, Olivetti Christian E, Desimone Martin F
IQUIMEFA-CONICET. Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires, Junin 956, Piso 3° (1113), Buenos Aires, Argentina.
Curr Pharm Biotechnol. 2015;16(7):661-7. doi: 10.2174/138920101607150427113355.
Non-porous bare silica nanoparticles, amine modified silica nanoparticles and mesoporous particles, were evaluated as carriers for sodium ibandronate. The synthesized nanoparticles were characterized by SEM, TEM, DLS and porosity. Then, their capacity to incorporate a bisphosphonate drug (sodium ibandronate) and the in vitro release behavior was analyzed by capillary electrophoresis. Mesoporous and amine-modified particles showed higher levels of drug incorporation, 44.68 mg g(-1) and 28.90 mg g(-1), respectively. The release kinetics from the two types of particles was similar following a first order kinetics. However, when these particles were included into collagen hydrogels only mesoporous nanoparticles had a sustained release for over 10 days. The biocompatibility of mesoporous particles towards Saos-2 cells was also evaluated by the MTT assay observing an increase in cell viability for concentrations lower than 0.6 mg ml(-1) of particles and a decrease for concentrations over 1.2 mg ml(-1). Furthermore, when these particles were incubated with mesenchymal cells it was observed that they had the capacity to promote the differentiation of the cells with a significant increase in the alkaline phosphatase activity.
对无孔裸二氧化硅纳米颗粒、胺修饰二氧化硅纳米颗粒和介孔颗粒作为伊班膦酸钠载体进行了评估。通过扫描电子显微镜（SEM）、透射电子显微镜（TEM）、动态光散射（DLS）和孔隙率对合成的纳米颗粒进行了表征。然后，通过毛细管电泳分析了它们负载双膦酸盐药物（伊班膦酸钠）的能力以及体外释放行为。介孔颗粒和胺修饰颗粒显示出较高的药物负载量，分别为44.68 mg g⁻¹和28.90 mg g⁻¹。两种颗粒的释放动力学遵循一级动力学，较为相似。然而，当将这些颗粒包封在胶原蛋白水凝胶中时，只有介孔纳米颗粒具有超过10天的持续释放。还通过MTT法评估了介孔颗粒对Saos-2细胞的生物相容性，观察到当颗粒浓度低于0.6 mg ml⁻¹时细胞活力增加，而当浓度超过1.2 mg ml⁻¹时细胞活力下降。此外，当这些颗粒与间充质细胞一起孵育时，观察到它们有促进细胞分化的能力，碱性磷酸酶活性显著增加。
