The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, No. 109, Xueyuanxi Road, Wenzhou, Zhejiang, China; Tongde Hospital of Zhejiang Province, No. 234, Gucui Road, Hangzhou, Zhejiang, China.
The Second Hospital Affiliated to Anhui University of Chinese Medicine, No.300, Shouchun Road, Hefei, Anhui, China.
Behav Brain Res. 2021 Mar 5;401:113085. doi: 10.1016/j.bbr.2020.113085. Epub 2020 Dec 28.
Rehabilitation training is routine for children who experience stroke, but its protective mechanism remains unclear. To study the effect of treadmill training intensity on hippocampal synaptic plasticity after cerebral ischemia, a model of middle cerebral artery occlusion (MCAO)/reperfusion was established in young rats to simulate childhood ischemic stroke. The rats were randomly allocated into five groups: sham operation, MCAO, low-intensity exercise and MCAO (5 m/min), medium-intensity exercise and MCAO (10 m/min), and high-intensity exercise and MCAO (15 m/min). Intervention was continued for 14 days, and a series of experimental tests were conducted. After MCAO, the juvenile rats exhibited a series of morphological and functional alterations, including changes in their neurobehavior and cerebral infarct volumes. Compared with control rats, MCAO rats had a longer escape latency and crossed fewer platforms in the water maze test and exhibited decreased hippocampal neuron density and Synapsin I and PSD95 expression. Furthermore, MCAO rats exhibited synapse morphology changes and abnormal serum levels of lactic acid and corticosterone. Treadmill training effectively reduced the neurobehavioral scores and cerebral infarction volumes, with medium-intensity training showing the best effect. Treadmill training shortened the escape latency, increased the number of platform crossings, and improved the spatial cognitive abilities of the rats, with the medium intensity training having the best effect on spatial learning/memory efficiency. Treadmill training increased the neuron density in the hippocampus, with the medium-intensity training resulting in the highest density. Treadmill training had a positive effect on the expression of Synapsin I and PSD95, with the medium-intensity training showing the strongest effect. Treadmill training improved the sub-microstructure synapse morphology, with the medium-intensity training demonstrating the best effect. Treadmill training increased the plasma levels of lactic acid and corticosterone, with the high-intensity training having the most obvious effect. Treadmill training can provide neuroprotection by promoting hippocampal synaptic plasticity, with medium-intensity training showing the most optimal effects.
康复训练是经历中风的儿童的常规治疗方法，但它的保护机制尚不清楚。为了研究跑步机训练强度对大脑中动脉阻塞（MCAO）/再灌注后海马突触可塑性的影响，建立了幼鼠大脑中动脉阻塞模型以模拟儿童期缺血性中风。将大鼠随机分为五组：假手术、MCAO、低强度运动和 MCAO（5m/min）、中强度运动和 MCAO（10m/min）、高强度运动和 MCAO（15m/min）。干预持续 14 天，并进行了一系列实验测试。MCAO 后，幼鼠表现出一系列形态和功能改变，包括神经行为和脑梗死体积的改变。与对照大鼠相比，MCAO 大鼠在水迷宫测试中逃避潜伏期更长，穿过平台的次数更少，海马神经元密度以及突触素 I 和 PSD95 表达降低。此外，MCAO 大鼠还表现出突触形态改变和血清乳酸和皮质酮水平异常。跑步机训练有效降低了神经行为评分和脑梗死体积，中等强度训练效果最佳。跑步机训练缩短了逃避潜伏期，增加了平台穿越次数，提高了大鼠的空间认知能力，中等强度训练对空间学习/记忆效率的影响最大。跑步机训练增加了海马神经元密度，中等强度训练的密度最高。跑步机训练对突触素 I 和 PSD95 的表达有积极影响，中等强度训练的效果最强。跑步机训练改善了亚微观结构突触形态，中等强度训练的效果最佳。跑步机训练增加了血浆中乳酸和皮质酮的水平，高强度训练的效果最明显。跑步机训练通过促进海马突触可塑性提供神经保护，中等强度训练的效果最佳。
