微藻在光自养批式培养过程中外源和内源酚的产生。

Extracellular and intracellular phenol production by microalgae during photoautotrophic batch cultivation.

机构信息

University Sapienza of Rome, Department of Chemistry, Piazzale Aldo Moro 5, 00185, Rome, Italy.

出版信息

N Biotechnol. 2021 May 25;62:1-9. doi: 10.1016/j.nbt.2020.12.003. Epub 2020 Dec 22.

DOI:10.1016/j.nbt.2020.12.003

Abstract

Understanding the mechanisms of phenol production by microalgae can contribute to the development of microalgal biorefinery processes with higher economic and environmental sustainability. However, little is known about how phenols are produced and accumulate during microalgal cultivation. In this study, both extracellular and intracellular phenol production by two microalgal strains (Tetradesmus obliquus and Chlorella sp.) were investigated throughout a conventional photoautotrophic batch cultivation. The highest intracellular phenol content (10-25 mg g) and productivity (12-18 mg L d) were attained for both strains in the first part of the batch, indicating a positive relation with nutrient availability and biomass productivity. Extracellular phenol production was 2-20 fold lower than intracellular phenols, but reached up to 27 mg L for T. obliquus and 13 mg L for Chlorella sp. The latter finding highlights relevant issues about the management of the exhausted culture medium, due to likely antimicrobial effects.

摘要

了解微藻产生酚类物质的机制有助于开发具有更高经济和环境可持续性的微藻生物炼制工艺。然而，对于微藻培养过程中酚类物质的产生和积累方式，人们知之甚少。在这项研究中，我们在常规的光自养分批培养过程中研究了两种微藻菌株（斜生栅藻和绿球藻）的胞外和胞内酚类物质的产生情况。在分批培养的前半部分，两种菌株的胞内酚含量（10-25mg/g）和产率（12-18mg/L/d）达到最高，这表明其与营养物质的可用性和生物量生产力呈正相关。胞外酚的产生量比胞内酚低 2-20 倍，但对于斜生栅藻可达到 27mg/L，对于绿球藻则达到 13mg/L。这一发现凸显了管理废弃培养液的重要性，因为其可能具有抗菌作用。