School of Marine, Fisheries, and Life Science, Pukyong National University, Busan 48513, Republic of Korea.
School of Marine, Fisheries, and Life Science, Pukyong National University, Busan 48513, Republic of Korea.
J Biotechnol. 2021 Jan 20;326:40-47. doi: 10.1016/j.jbiotec.2020.12.009. Epub 2020 Dec 29.
The optimal conditions for high biomass and lipid production from Nannochloris atomus were evaluated. The parameters used in this study were light emitting diode (LED) wavelength mixing ratio, the photoperiod, salinity tolerance, and single and combined stresses. Biomass production was monitored in the first phase using red LED (625 nm), followed by lipid production by green LED (520 nm) in the second phase. The optimal conditions were obtained using a single red LED with light:dark durations of 20:4 h and two days of exposure in combined stresses of 1.06 M NaCl and green LED. Under these conditions, 68.6 % (w/w) lipid content were obtained. Compared to the non-stress control, the lipid content was increased by 31.9 %. Linolenic acid (C18:3) the omega-3 fatty acid was produced up to 52.4 % in 1.06 M NaCl as a single stress.
评价了从念珠藻属中获得高生物质和脂质产量的最佳条件。本研究中使用的参数为发光二极管（LED）波长混合比、光周期、盐度耐受性以及单一和复合胁迫。在第一阶段使用红色 LED（625nm）监测生物质产量，然后在第二阶段使用绿色 LED（520nm）进行脂质生产。在单一红色 LED 下，光暗时间为 20:4 h，在 1.06 M NaCl 和绿色 LED 的复合胁迫下暴露两天，可获得最佳条件。在这些条件下，获得了 68.6%（w/w）的脂质含量。与非胁迫对照相比，脂质含量增加了 31.9%。在 1.06 M NaCl 作为单一胁迫下，ω-3 脂肪酸亚麻酸（C18:3）的产量最高可达 52.4%。
