Department of Biotechnology, Pukyong National University, Busan 48513, Republic of Korea.
Busan Techno-park Marine Bio-industry Development Center (MBDC), Busan 46048, Republic of Korea.
Bioresour Technol. 2016 Oct;218:1279-83. doi: 10.1016/j.biortech.2016.07.078. Epub 2016 Jul 21.
The effects of light-emitting diode (LED) wavelength, light intensity, nitrate concentration, and time of exposure to different LED wavelength stresses in a two-phase culture on lipid production were evaluated in the microalga, Picochlorum atomus. The biomass produced by red LED light was higher than that produced by purple, blue, green, or yellow LED and fluorescent lights from first phase of two-phase culture. The highest lipid production of P. atomus was 50.3% (w/w) with green LED light at 2days of second phase as light stress. Fatty acid analysis of the microalgae showed that palmitic acid (C16:0) and linolenic acid (C18:3) accounted for 84-88% (w/w) of total fatty acids from P. atomus. The two-phase culture of P. atomus is suitable for biofuel production due to higher lipid productivity and favorable fatty acid composition.
研究了在两相培养中，发光二极管（LED）波长、光强、硝酸盐浓度以及暴露于不同 LED 波长应激的时间对微藻原甲藻脂质生产的影响。在两相培养的第一阶段，与紫光、蓝光、绿光或黄光 LED 和荧光灯相比，红光 LED 产生的生物量更高。在第二阶段的第 2 天，绿光 LED 的光胁迫下，原甲藻的最高脂质产量为 50.3%（w/w）。微藻的脂肪酸分析表明，棕榈酸（C16:0）和亚麻酸（C18:3）占原甲藻总脂肪酸的 84-88%（w/w）。由于较高的脂类生产力和有利的脂肪酸组成，原甲藻的两相培养适合生物燃料生产。
