González-Stegmaier Roxana, Peña Andrea, Villarroel-Espíndola Franz, Aguila Patricia, Oliver Cristian, MacLeod-Carey Desmond, Rozas-Serri Marco, Enriquez Ricardo, Figueroa Jaime
Laboratorio de Biología Molecular de Peces, Instituto de Bioquímica y Microbiología, Universidad Austral de Chile, Valdivia, Chile; Laboratorio Medicina Traslacional. Instituto Clínico Oncológico. Fundación Arturo López Pérez, Santiago, Chile.
Laboratorio Pathovet Ltda, Puerto Montt, Chile.
Dev Comp Immunol. 2021 Apr;117:103988. doi: 10.1016/j.dci.2020.103988. Epub 2020 Dec 23.
Flagellin is the major component of the flagellum, and a ligand for Toll-like receptor 5. As reported, recombinant flagellin (rFLA) from Vibrio anguillarum and its D1 domain (rND1) are able to promote in vitro an upregulation of pro-inflammatory genes in gilthead seabream (Sparus aurata) and rainbow trout (Oncorhynchus mykiss) macrophages. This study evaluated the in vitro and in vivo stimulatory/adjuvant effect for rFLA and rND1 during P. salmonis vaccination in Atlantic salmon (Salmo salar). We demonstrated that rFLA and rND1 are molecules able to generate an acute upregulation of pro-inflammatory cytokines (IL-1β, IL-8, IL-12β), allowing the expression of genes associated with T-cell activation (IL-2, CD4, CD8β), and differentiation (IFNγ, IL-4/13, T-bet, Eomes, GATA3), in a differential manner, tissue/time dependent way. Altogether, our results suggest that rFLA and rND1 are valid candidates to be used as an immuno-stimulant or adjuvants with existing vaccines in farmed salmon.
鞭毛蛋白是鞭毛的主要成分，也是Toll样受体5的配体。据报道，鳗弧菌的重组鞭毛蛋白（rFLA）及其D1结构域（rND1）能够在体外促进金头鲷（Sparus aurata）和虹鳟（Oncorhynchus mykiss）巨噬细胞中促炎基因的上调。本研究评估了rFLA和rND1在大西洋鲑（Salmo salar）感染鲑居尾孢虫疫苗接种过程中的体外和体内刺激/佐剂作用。我们证明，rFLA和rND1能够以不同的方式、组织/时间依赖性方式急性上调促炎细胞因子（IL-1β、IL-8、IL-12β），使与T细胞活化（IL-2、CD4、CD8β）和分化（IFNγ、IL-4/13、T-bet、Eomes、GATA3）相关的基因得以表达。总之，我们的结果表明，rFLA和rND1是作为养殖鲑鱼现有疫苗的免疫刺激剂或佐剂的有效候选物。
