College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang 453003, China.
College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang 453003, China.
Gen Comp Endocrinol. 2021 Mar 1;303:113705. doi: 10.1016/j.ygcen.2020.113705. Epub 2020 Dec 24.
The present study aimed to investigate the effect of ghrelin on the degree of bursa of Fabricius (BF) fibrosis in infectious bursal disease virus-infected chickens. Specific pathogen free (SPF) chicks were divided into four groups. One group was used as the control ("C"). The other three groups were inoculated with IBDV on the 19th day, of which two were injected intraperitoneally with 0.5 nmol ("LG") or 1.0 nmol ("HG") ghrelin/100 g weight from the 18th day to the 22nd day, and one was injected intraperitoneally with PBS ("I"). Hematoxylin-eosin staining, Masson's staining, and quantitative real-time PCR were used to determine the effects of ghrelin on the degree of inflammatory cell infiltration, the bursal fibrosis degree, and the expression of TGF-β and MMP-9 mRNA in IBDV-infected SPF chicks. The results showed that ghrelin administration reduced the number of infiltrated inflammatory cells in BF from 5 dpi and significantly attenuated the degree of fibrosis induced by IBDV from 2 dpi to 7 dpi (P < 0.05). Moreover, the TGF-β expression in the LG and HG groups were significantly or highly significantly lower (P < 0.05 or P < 0.01) than those of I group from 2 dpi to 5 dpi. In addition, ghrelin administration downregulated MMP-9 expression evoked by IBDV from 2 dpi to 7 dpi (P < 0.05 or P < 0.01). These results suggested that ghrelin attenuated the bursal fibrosis degree of IBDV-infected SPF chicks by reducing the number of inflammatory cells and by decreasing the expression of TGF-β and MMP-9, which shortened the process of bursa recovery.
本研究旨在探讨 ghrelin 对传染性法氏囊病病毒感染鸡法氏囊纤维化程度的影响。无特定病原体（SPF）鸡雏分为四组。一组用作对照（“C”）。另外三组于第 19 天接种 IBDV，其中两组分别于第 18 天至第 22 天腹腔内注射 0.5 nmol（“LG”）或 1.0 nmol（“HG”）ghrelin/100 g 体重，一组腹腔内注射 PBS（“I”）。使用苏木精-伊红染色、马松染色和实时定量 PCR 来确定 ghrelin 对 IBDV 感染 SPF 鸡雏炎症细胞浸润程度、法氏囊纤维化程度以及 TGF-β 和 MMP-9 mRNA 表达的影响。结果表明，ghrelin 给药可减少 BF 中浸润的炎症细胞数量，从 5 dpi 开始，并显著减轻 IBDV 诱导的纤维化程度，从 2 dpi 持续到 7 dpi（P < 0.05）。此外，LG 和 HG 组的 TGF-β 表达从 2 dpi 到 5 dpi 均显著或高度显著低于 I 组（P < 0.05 或 P < 0.01）。此外，ghrelin 给药可下调 IBDV 从 2 dpi 到 7 dpi 诱导的 MMP-9 表达（P < 0.05 或 P < 0.01）。这些结果表明，ghrelin 通过减少炎症细胞数量和降低 TGF-β 和 MMP-9 的表达来减轻 IBDV 感染 SPF 鸡雏的法氏囊纤维化程度，从而缩短法氏囊恢复的过程。
