State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Animal Husbandry and Veterinary Sciences, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
Viruses. 2022 Jul 25;14(8):1615. doi: 10.3390/v14081615.
The bursa of Fabricius is an immunologically organ against the invasion of duck reovirus (DRV), which is a fatal bird virus belonging to the Reoviridae family. However, responses of the bursa of Fabricius of Cairna moschata to novel DRV (NDRV) infection are largely unknown. Transcriptomes and proteomes of the samples from control and two NDRV strain (HN10 and JDm10) with different virulence were analyzed. Differentially expressed genes and differential accumulated proteins were enriched in the serine protease system and innate immune response clusters. Most of the immune-related genes were up-regulated under both JDm10/HN10 infections. However, the immune-related proteins were only accumulated under HN10 infection. For the serine protease system, coagulation factor IX, three chains of fibrinogen, and complements C8, C5, and C2s were significantly up-regulated by the HN10 infection, suggesting that the serine protease-mediated immune system might be involved in the resistance to NDRV infection. For the innate and adaptive immune system, RIG-I, MDA5, MAPK20, and IRF3 were significantly up-regulated, indicating their important roles against invaded virus. TLR-3 and IKBKB were only up-regulated in the liver cells, MAPK20 was only up-regulated in the bursa of Fabricius cells, and IRAK2 was only up-regulated in the spleen samples. Coagulation factor IX was increased in the bursa of Fabricius, not in the liver and spleen samples. The data provides a detailed resource for studying the proteins participating in the resistances of the bursa of Fabricius of duck to NDRV infections.
法氏囊是一种免疫器官，可抵御鸭呼肠孤病毒（DRV）的侵袭，该病毒是一种属于呼肠孤病毒科的致命鸟类病毒。然而，关于新型 DRV（NDRV）感染对麝香鸭法氏囊的影响，我们知之甚少。本研究对来自对照组和两株具有不同毒力的 NDRV （HN10 和 JDm10）的样本的转录组和蛋白质组进行了分析。差异表达基因和差异积累蛋白富集于丝氨酸蛋白酶系统和固有免疫反应簇。JDm10/HN10 感染下，大多数免疫相关基因均上调。然而，仅在 HN10 感染下才会积累免疫相关蛋白。对于丝氨酸蛋白酶系统，凝血因子 IX、纤维蛋白原的三条链和补体 C8、C5 和 C2s 显著上调，表明丝氨酸蛋白酶介导的免疫系统可能参与了对 NDRV 感染的抵抗。对于固有和适应性免疫系统，RIG-I、MDA5、MAPK20 和 IRF3 显著上调，表明它们在对抗入侵病毒方面的重要作用。TLR-3 和 IKBKB 仅在肝细胞中上调，MAPK20 仅在法氏囊中上调，IRAK2 仅在脾脏样本中上调。凝血因子 IX 在法氏囊中增加，而不在肝脏和脾脏样本中增加。该数据为研究鸭法氏囊抵抗 NDRV 感染的参与蛋白提供了详细的资源。
