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胡萝卜生物废料中提取的亚麻籽油中的类胡萝卜素乳液的三因素方差分析。

Three way ANOVA for emulsion of carotenoids extracted in flaxseed oil from carrot bio-waste.

机构信息

Dairy Technology Division, ICAR-National Dairy Research Institute, Karnal 132001, Haryana, India.

Dairy Technology Division, ICAR-National Dairy Research Institute, Karnal 132001, Haryana, India.

出版信息

Waste Manag. 2021 Feb 15;121:67-76. doi: 10.1016/j.wasman.2020.11.037. Epub 2020 Dec 22.

DOI:10.1016/j.wasman.2020.11.037
PMID:33360169
Abstract

The juice expelled from carrot, a globally produced root vegetable, leavesbehind carrot pomace (a bio- and horticultural waste) which is potentially rich source of micro-nutrients and carotenoids.However, it is discarded as waste or used as animal feed. It holds potential to be channelized to food chain by a couple of technological interventions. In this regard, present work was aimed at preparing stable emulsion based delivery system for 'green' carotenoids extracted from carrot-pomace in flaxseed oil (a green solvent), and at maximizing the amount of core material so that the resultant emulsion can potentially be used as a source of both carotenoids and omega-3 fatty acid of flaxseed oil origin. The study used natural emulsifier. Preparation of oil-in-water emulsion was optimized using 3 factorial experiment by varying levels of extract containing carotenoid (30-40%), whey protein concentrates (WPC-80) and lactose. The optimized emulsion (CREm) was selected on the basis of particle size, zeta potential, color values (L*, a*, b*) and viscosity statistically analyzed via three-way ANOVA using Proc GLM of SAS 9.3 (described in detail in this paper); the respective values of these parameters being 120.03 ± 8.20 nm, -16.57 ± 0.49 mV, 75.11 ± 0.04, 9.66 ± 0.32, 50.29 ± 0.62, and 0.124 ± 0.0115 Pa.s for CREm. CREm contained 35% flaxseed oil, 10% WPC-80 and 5% lactose and showed good centrifugal and gravitational stability (15 days). It was analyzed for total carotenoid content, antioxidant activities (ABTS (2,2-azinobis-(3-ethylbenzthiazoline-6sulfonic acid), DPPH (2,2-Diphenyl-1-picrylhydrazyl) and FRAP (Ferric reducing antioxidant power assay)) and microstructure.

摘要

从全球生产的根茎类蔬菜胡萝卜中榨出的汁液会留下胡萝卜渣(一种生物和园艺废物),而胡萝卜渣中富含微量营养素和类胡萝卜素。然而,它通常被当作废物丢弃,或用作动物饲料。通过一些技术干预,它有可能被纳入食物链。在这方面,目前的工作旨在以亚麻籽油(一种绿色溶剂)为基础,从胡萝卜渣中提取“绿色”类胡萝卜素,制备稳定的乳液输送系统,并使核心材料的含量最大化,以便所得乳液可潜在地用作胡萝卜素和亚麻籽油来源的欧米伽-3 脂肪酸的来源。该研究使用天然乳化剂。通过在 30-40%含类胡萝卜素的提取物、乳清蛋白浓缩物(WPC-80)和乳糖的水平上变化,使用 3 因子实验优化水包油乳液的制备。通过使用 SAS 9.3 的 Proc GLM 进行三向方差分析(在本文中详细描述),根据粒径、Zeta 电位、颜色值(L*、a*、b*)和粘度对优化后的乳液(CREm)进行统计学分析,选择最佳乳液(CREm);这些参数的相应值为 120.03±8.20nm、-16.57±0.49mV、75.11±0.04、9.66±0.32、50.29±0.62 和 0.124±0.0115Pa.s。CREm 含有 35%的亚麻籽油、10%的 WPC-80 和 5%的乳糖,具有良好的离心和重力稳定性(15 天)。对其总类胡萝卜素含量、抗氧化活性(ABTS(2,2-联氮-双-(3-乙基苯并噻唑啉-6-磺酸))、DPPH(2,2-二苯基-1-苦基肼)和 FRAP(铁还原抗氧化能力测定法))和微观结构进行了分析。

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