College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China; Key Laboratory of the Provincial Education, Department of Heilongjiang for Common Animal Disease Prevention and Treatment, Northeast Agricultural University, Harbin 150030, China.
College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China; Key Laboratory of the Provincial Education, Department of Heilongjiang for Common Animal Disease Prevention and Treatment, Northeast Agricultural University, Harbin 150030, China.
Res Vet Sci. 2021 Jan;134:127-136. doi: 10.1016/j.rvsc.2020.12.004. Epub 2020 Dec 13.
Layer fatigue syndrome caused by the lack of calcium and phosphorus can cause fracture in laying hens. The effect of phosphorus deficiency on the femur of laying hens with layer fatigue syndrome has not been studied. In this study, sixty 22-week-old Roman white layers were randomly divided into control group (group C) and low phosphorus group (group P), 30 individuals in each group. The available phosphorus content of group P was 0.18%. At the age of 26, 30 and 34 weeks, the production performance, biomechanical index, protein expression, histopathological change of femur and serological index were detected. The results showed that the laying rate, egg quality and body weight of laying hens, bone density, cortical bone thickness, rigidity, flexural modulus, flexural rigidity, the maximum load of femur and expression of osteocalcin (OCN), receptor activator of nuclear factor kappa-Β (RANK) and receptor activator of nuclear factor kappa-Β ligand (RANKL) decreased of group P. The number of osteocytes was decreased, and the voids was increased. However, cell lacunae were not obvious. The levels of phosphorus, calcium and OCN were increased, and the content of estradiol (E), OPG and calcitonin (CT) were decreased in serum. In conclusion, the low phosphorus diet can induce layer fatigue syndrome and affect the content of OPG and E in serum and the expression of OCN, OPG, RANK and RANKL in femur protein, which leads to the imbalance of bone homeostasis, the thinning of femur cortex bone and the decrease of bone density.
由钙磷缺乏引起的乏钙症可导致产蛋鸡发生骨折。目前尚未研究磷缺乏对产蛋疲劳症母鸡股骨的影响。本研究选择 60 只 22 周龄罗曼白产蛋鸡，随机分为对照组（C 组）和低磷组（P 组），每组 30 只。P 组饲粮有效磷含量为 0.18%。在 26、30 和 34 周龄时，检测产蛋性能、生物力学指标、蛋白表达、股骨组织病理学变化和血清生化指标。结果表明：与 C 组相比，P 组产蛋率、蛋品质和体重、骨密度、皮质骨厚度、刚性、弯曲模量、弯曲刚度、股骨最大载荷和骨钙素（OCN）、核因子 κB 受体激活剂（RANK）和核因子 κB 受体激活剂配体（RANKL）表达降低，骨细胞数量减少，骨陷窝增多，骨小梁间隙增大，但细胞陷窝不明显。血清磷、钙和 OCN 含量增加，雌二醇（E）、骨保护素（OPG）和降钙素（CT）含量降低。综上所述，低磷饲粮可诱导产蛋疲劳症，影响血清 OPG 和 E 含量以及股骨 OCN、OPG、RANK 和 RANKL 蛋白表达，导致骨稳态失衡、股骨皮质骨变薄和骨密度降低。
