石莼在海洋养殖沉积物中微生物群落和抗生素抗性基因演化中的作用。
The role of Ulva fasciata in the evolution of the microbial community and antibiotic resistance genes in maricultural sediments.
机构信息
Department of Environmental Science and Engineering, Zhejiang Ocean University, Zhoushan City, Zhejiang Province, People's Republic of China.
Department of Environmental Science and Engineering, Zhejiang Ocean University, Zhoushan City, Zhejiang Province, People's Republic of China.
出版信息
Mar Pollut Bull. 2021 Feb;163:111940. doi: 10.1016/j.marpolbul.2020.111940. Epub 2020 Dec 24.
This study explored changes in the microbial community and antibiotic resistance genes (ARGs) in maricultural clam sediment after 3-month co-culture with different densities (0, 5 and 12 g L) of seaweed Ulva fasciata (U. fasciata). The maximum removal rates of NO-N, PO-P, and inhibition of Vibrio culturability occurred at presence of 12 g LU. fasciata. A significant decrease by 14.0% of the total ARGs was found in control sediment without U. fasciata after separation from the original niches, while the total ARGs further increased by 5.58%and 4.65% at presence of 5 and 12 g L of U. fasciata in compared with control sediment, respectively, strongly related with Chloroflexi, Spirochaetes, Proteobacteria and Bacteroidetes hosts. In addition, U. fasciata favored the decline of absolute gene numbers of some tetracycline resistance genes (tetPB, tetW, otrA, tetT, tetO) and class 1 integron-integrase gene.
本研究探讨了在与不同密度（0、5 和 12 g/L）的海藻石莼（U. fasciata）共生 3 个月后，养殖蛤沉积物中微生物群落和抗生素耐药基因（ARGs）的变化。在存在 12 g/L U. fasciata 的情况下，NO-N、PO-P 的最大去除率和弧菌可培养性的抑制率最高。在与原始小生境分离后，无 U. fasciata 的对照沉积物中的总 ARGs 显著减少了 14.0%，而在存在 5 和 12 g/L U. fasciata 的情况下，总 ARGs 分别比对照沉积物进一步增加了 5.58%和 4.65%，与绿弯菌门、螺旋体门、变形菌门和拟杆菌门宿主密切相关。此外，U. fasciata 有利于一些四环素耐药基因（tetPB、tetW、otrA、tetT、tetO）和类 1 整合子整合酶基因的绝对基因数量的下降。
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