Radioisotopes Department, Nuclear Research Centre, Atomic Energy Authority, Dokki, Giza, Egypt.
Botany Department, Faculty of Science, Ain Shams University, Abbassia Square, Cairo, 11566, Egypt.
Environ Sci Pollut Res Int. 2021 Feb;28(6):6830-6842. doi: 10.1007/s11356-020-11036-z. Epub 2020 Oct 3.
Cardiovascular diseases are key complications primarily associated with hyperthyroidism disorders. The present study sought to ameliorate hyperthyroidism-mediated cardiovascular inflammations and related oxidative stress paradigms in experimental rats using the broadly distributed green seaweed Ulva fasciata. Forty-eight adult male albino rats were recruited and randomly classified into six groups. Hyperthyroidism was stimulated using L-thyroxine sodium at a dose of 100 μg/kg i.p. for 3 weeks daily. Further, 200 mg/kg b.wt. concentration of the U. fasciata methanolic (U. fasciata-MeOH) extract was the recommended dose and administrated orally to the hyperthyroid rats. The standard commercial drug "propranolol hydrochloride" was also tested at a dose of 10 mg/kg i.p. to compare the findings obtained from the seaweed extract. A combined treatment with the U. fasciata-MeOH extract and propranolol hydrochloride was also assessed. Our results implied that the treatment of hyperthyroid rats with the U. fasciata-MeOH extract significantly reduced serum levels of the thyroid hormones T3 and T4, proinflammatory cytokines (TNF-α, MPO, and CRP), triglycerides and total cholesterol, as well as the cardiac biomarkers CK-MB, LDH, and troponin to thresholds close to those of the standard drug. In addition, levels of high-density lipoprotein cholesterol (HDL-C) and interleukin 10 (IL-10) were significantly upregulated. Hyperthyroid rats only treated with propranolol hydrochloride, or with a combination of the drug and the seaweed extract, conferred the same observations. Histopathological architecture boosted our interesting findings where the myocardium tissues in hyperthyroid rats, administrated the U. fasciata-MeOH extract or/and propranolol hydrochloride, exhibited more or less a normal structure as the control, reflecting the potential cardiovascular recovery exerted by this seaweed extract. In vitro DPPH, ABTS, and FRAP antioxidant assays of the U. fasciata-MeOH extract showed an outstanding ROS-scavenging potential. HPLC analysis of the U. fasciata-MeOH extract unraveled an inestimable valuable array of phenolics (mainly p-coumaric, gallic, ferulic, chlorogenic, and syringic acids) and flavonoids (hesperidin, kaempferol, catechin, quercetin, and rutin). Conclusively, the seaweed U. fasciata is a profitable source of antioxidant polyphenolics characterized by having a pharmaceutical potential against hyperthyroidism-linked cardiovascular inflammations and oxidative stress patterns due to their substantial free radical quenching properties, and also via regulating the signalling pathways of the proinflammatory, lipid profile, and cardiac biomarkers.
心血管疾病是与甲状腺功能亢进症相关的主要并发症。本研究旨在使用广泛分布的绿藻石莼（Ulva fasciata）来改善实验大鼠的甲状腺功能亢进介导的心血管炎症和相关氧化应激模式。招募了 48 只成年雄性白化大鼠，并将其随机分为六组。使用 100μg/kg 的 L-甲状腺素钠每天腹腔注射 3 周来刺激甲状腺功能亢进。此外，200mg/kg 的石莼甲醇提取物（U. fasciata-MeOH）浓度为推荐剂量，并口服给予甲状腺功能亢进大鼠。还测试了标准商业药物盐酸普萘洛尔（propranolol hydrochloride），剂量为 10mg/kg 腹腔注射，以比较从海藻提取物中获得的发现。还评估了 U. fasciata-MeOH 提取物与盐酸普萘洛尔的联合治疗。我们的结果表明，用 U. fasciata-MeOH 提取物治疗甲状腺功能亢进大鼠可显著降低血清甲状腺激素 T3 和 T4、促炎细胞因子（TNF-α、MPO 和 CRP）、甘油三酯和总胆固醇以及心肌标志物 CK-MB、LDH 和肌钙蛋白的水平接近标准药物的阈值。此外，高密度脂蛋白胆固醇（HDL-C）和白细胞介素 10（IL-10）的水平显著上调。仅用盐酸普萘洛尔治疗的甲状腺功能亢进大鼠，或用药物和海藻提取物联合治疗的大鼠，也有相同的观察结果。心肌组织的组织病理学结构增强了我们的有趣发现，用 U. fasciata-MeOH 提取物或/和盐酸普萘洛尔治疗的甲状腺功能亢进大鼠的心肌组织或多或少呈现正常结构，反映了这种海藻提取物对心血管恢复的潜在作用。U. fasciata-MeOH 提取物的 DPPH、ABTS 和 FRAP 抗氧化测定表明其具有出色的 ROS 清除潜力。U. fasciata-MeOH 提取物的 HPLC 分析揭示了大量宝贵的酚类（主要是对香豆酸、没食子酸、阿魏酸、绿原酸和丁香酸）和类黄酮（柚皮苷、山柰酚、儿茶素、槲皮素和芦丁）。总之，海藻 U. fasciata 是一种富含抗氧化多酚的有益来源，具有治疗甲状腺功能亢进相关心血管炎症和氧化应激模式的药用潜力，这归因于其强大的自由基清除特性，以及通过调节促炎、脂质谱和心肌标志物的信号通路。
