采用气相色谱-质谱联用技术同时测定啤酒中的乙二醇和二甘醇。
Simultaneous quantification of ethylene glycol and diethylene glycol in beer by gas chromatography coupled to mass spectrometry.
机构信息
Faculdade de Farmácia, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-901, Brazil; Laboratório Federal de Defesa Agropecuária, Avenida Raja Gabaglia, 245, Setor H, Bairro Cidade Jardim, Belo Horizonte, MG 30380-103, Brazil.
Laboratório Federal de Defesa Agropecuária, Avenida Raja Gabaglia, 245, Setor H, Bairro Cidade Jardim, Belo Horizonte, MG 30380-103, Brazil.
出版信息
Food Chem. 2021 Jun 1;346:128871. doi: 10.1016/j.foodchem.2020.128871. Epub 2020 Dec 15.
An analytical method for the simultaneous determination of ethylene glycol and diethylene glycol in beer was developed and validated according to current legislation. This method includes the application of sample dilution with ethanol followed by quantification using gas chromatography coupled to mass spectrometry. All figures of merit were within the limits established by regulation. The recoveries of the analytes, expressed as mean recovery, were between 91.9% and 108.9%. Precision, in terms of repeatability and intermediate precision, was established (relative standard deviations were lower than or equal to 10%). The limits of detection (10.0 and 5.0 mg.L for ethylene glycol and diethylene glycol, respectively) and quantification (15.0 mg.L for ethylene glycol and diethylene glycol) obtained were appropriate. Finally, the present method was applied for determination of ethylene glycol and diethylene glycol in 701 beer samples (from 67 different brands and 128 different labels), proving to be reliable.
建立并验证了一种根据现行法规同时测定啤酒中乙二醇和二甘醇的分析方法。该方法包括用乙醇稀释样品，然后用气相色谱-质谱联用进行定量。所有的度量值都在规定的范围内。分析物的回收率（表示为平均回收率）在 91.9%至 108.9%之间。精密度（重复性和中间精密度）也得到了确定（相对标准偏差均低于或等于 10%）。检测限（乙二醇和二甘醇分别为 10.0 和 5.0 mg/L）和定量限（乙二醇和二甘醇均为 15.0 mg/L）均合适。最后，本方法应用于 701 个啤酒样品（来自 67 个不同品牌和 128 个不同标签）中乙二醇和二甘醇的测定，结果可靠。
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