Du Jinyan, Yang Ying, Shao Taili, Qi Shuangqing, Zhang Ping, Zhuo Shujuan, Zhu Changqing
Anhui Key Laboratory of Chemo-Biosensing, Key Laboratory of Functional Molecular Solids, Ministry of Education, College of Chemistry and Materials Science, Anhui Normal University, Wuhu, 241000, PR China.
Anhui Key Laboratory of Chemo-Biosensing, Key Laboratory of Functional Molecular Solids, Ministry of Education, College of Chemistry and Materials Science, Anhui Normal University, Wuhu, 241000, PR China.
J Colloid Interface Sci. 2021 Apr;587:376-384. doi: 10.1016/j.jcis.2020.11.108. Epub 2020 Dec 2.
A simple "OFF-ON" fluorescent system was proposed for selective and sensitive detection of ferric ion (Fe) and pyrophosphate (PPi) in living cells. The method was constructed based on the bright yellow emission of carbon dots (y-CDs), which were prepared using o-phenylenediamine (OPD) as the precursor via a facile hydrothermal treatment. The as-obtained y-CDs, with an average size of 2.6 nm, exhibited an excitation-independent emission peak at 574 nm. The fluorescence of y-CDs can be remarkably quenched by Fe with high selectivity and sensitivity. Interestingly, the quenched fluorescence can be recovered regularly upon addition of PPi, showing a promising detection for PPi. The linear ranges for Fe and PPi detections were 0.05-80 and 0.5-120 μM, respectively, and the corresponding limit of detections (LODs) were 22.1 and 73.9 nM. As we proved the y-CDs have negligible cytotoxicity and excellent biocompatibility, further application to the fluorescence imaging of intracellular Fe and PPi were conducted, suggesting the prepared y-CDs can be used to monitor Fe and PPi variation in living cells. Overall, our developed y-CDs-based OFF-ON switch fluorescent probe has the advantages of simplicity, agility, high sensitivity and selectivity, which provides a promising platform for environmental and biology applications, and paves a new avenue for monitoring the hydrolysis process of adenosine triphosphate disodium salt (ATP) by detection of PPi in organisms.
本文提出了一种简单的“开-关”荧光系统，用于选择性灵敏地检测活细胞中的铁离子（Fe）和焦磷酸（PPi）。该方法基于碳点（y-CDs）的亮黄色发射构建，y-CDs以邻苯二胺（OPD）为前驱体，通过简便的水热法制备。所制备的y-CDs平均尺寸为2.6 nm，在574 nm处呈现出不依赖于激发光的发射峰。y-CDs的荧光可被Fe高效选择性地猝灭。有趣的是，加入PPi后，猝灭的荧光可被恢复，有望用于PPi的检测。Fe和PPi检测的线性范围分别为0.05 - 80 μM和0.5 - 120 μM，相应的检测限（LOD）分别为22.1和73.9 nM。由于我们证明了y-CDs具有可忽略的细胞毒性和优异的生物相容性，因此进一步进行了细胞内Fe和PPi的荧光成像应用，表明所制备的y-CDs可用于监测活细胞中Fe和PPi的变化。总体而言，我们开发的基于y-CDs的开-关型荧光探针具有简单、灵敏、高灵敏度和高选择性的优点，为环境和生物学应用提供了一个有前景的平台，并为通过检测生物体内的PPi来监测三磷酸腺苷二钠盐（ATP）的水解过程开辟了一条新途径。
