Anhui Key Laboratory of Chemo-Biosensing, Key Laboratory of Functional Molecular Solids, Ministry of Education, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China.
Anhui Key Laboratory of Chemo-Biosensing, Key Laboratory of Functional Molecular Solids, Ministry of Education, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Mar 5;268:120681. doi: 10.1016/j.saa.2021.120681. Epub 2021 Nov 30.
High-performance determination of pyrophosphate ions (PPi) and pH is an important goal in biological systems. In this work, Cu-doped carbon dots (Cu-CDs) were synthesized rapidly and simply via a one-pot hydrothermal method. The as-obtained Cu-CDs, with an average size of 2.55 nm, exhibit an excitation-independent fluorescence emission and possess desirable functional groups of carboxyl and amine, which can be served as fluorescence nanoprobes for detection of PPi based on surface passivation. Under the optimal condition, the linear range for detection of PPi was 0.05-20 µM, and the corresponding limit of detection (LOD) was 0.013 µM, indicative of a promising assay for the PPi. Moreover, the fluorescent intensity of the Cu-CDs is linear against pH value from 6 to 8.7 in buffer solution, suggesting the feasibility as a pH sensor. The synthesized Cu-CDs coated fluorescent paper indeed can monitor pH in urine with satisfaction by naked eyes through ultraviolet irradiation. The successful detection of PPi and the visual detection of pH value suggest a highly promising application of Cu-CDs in the field of biosensing.
快速、简便地通过一锅水热法合成了铜掺杂碳点（Cu-CDs）。所得 Cu-CDs 的平均尺寸为 2.55nm，具有激发独立的荧光发射，并具有理想的羧基和胺基官能团，可作为基于表面钝化的 PPi 检测的荧光纳米探针。在最佳条件下，PPi 的检测线性范围为 0.05-20µM，相应的检测限（LOD）为 0.013µM，表明这是一种很有前途的 PPi 检测方法。此外，Cu-CDs 的荧光强度在缓冲溶液中 pH 值为 6 到 8.7 范围内呈线性关系，表明其作为 pH 传感器的可行性。合成的 Cu-CDs 涂层荧光纸确实可以通过紫外照射用肉眼满意地监测尿液中的 pH 值。成功检测到 PPi 和可视化检测 pH 值表明，Cu-CDs 在生物传感领域具有广阔的应用前景。
