CAS Key Laboratory of Biomedical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Science (CAS), Suzhou 215163, P. R. China.
Analyst. 2021 Jul 26;146(15):4954-4963. doi: 10.1039/d1an00828e.
Iron, nitrogen-co-doped carbon quantum dots (Fe,N-CDs) were prepared via a simple one-step hydrothermal method. The quantum yield of fluorescence reached about 27.6% and the blue-emissive Fe,N-CDs had a mean size of 3.76 nm. The as-prepared carbon quantum dots showed good solubility, a high quantum yield, good biocompatibility, low cytotoxicity, and high photostability. Interestingly, the as-prepared Fe,N-CDs exhibited good selectivity and sensitivity toward both hematin and ferric ions, and the limit of detection for hematin and ferric ions was calculated to be about 0.024 μM and 0.64 μM, respectively. At the same time, Fe,N-CDs were used for imaging HeLa cells and showed that most Fe,N-CDs were detained in the lysosome. Thus, this fluorescent probe has potential application in the quantitative detection of hematin or Fe3+ in a complex environment and for determining Fe3+ at the cellular level.
通过简单的一步水热法制备了铁、氮共掺杂碳量子点（Fe，N-CDs）。荧光量子产率约为 27.6%，蓝色发射的 Fe，N-CDs 的平均尺寸为 3.76nm。所制备的碳量子点具有良好的溶解性、高量子产率、良好的生物相容性、低细胞毒性和高光稳定性。有趣的是，所制备的 Fe，N-CDs 对血红素和铁离子均表现出良好的选择性和灵敏度，血红素和铁离子的检测限分别计算约为 0.024μM 和 0.64μM。同时，Fe，N-CDs 被用于 HeLa 细胞成像，结果表明大多数 Fe，N-CDs 被滞留在溶酶体中。因此，这种荧光探针在复杂环境中对血红素或 Fe3+的定量检测以及在细胞水平上测定 Fe3+具有潜在的应用。
