School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China.
School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China.
J Environ Manage. 2021 Mar 1;281:111896. doi: 10.1016/j.jenvman.2020.111896. Epub 2020 Dec 25.
In this study, the effects of ciprofloxacin on activated sludge were evaluated based on the microbial community and metabolic characteristics. The results indicated that the metabolism of chemical oxygen demand (COD) and nitrogen were inhibited with ciprofloxacin at mg/L level compared to the control experiment, and the concentration of ciprofloxacin was slightly decreased. High-throughput sequencing (HTS) results showed that ciprofloxacin greatly shaped the microbial communities in activated sludge, especially for the Nitrospirae phylum and Nitrospira genus. High concentrations of ciprofloxacin stimulated the enrichment of Zoogloea, thus reducing the stability of the activated sludge. Moreover, quinolone resistance proteins in Aeromonas were enriched, which demonstrates their competitive advantage in these enrichment incubations. Finally, the functional profiles were predicted through Tax4Fun, which revealed the adaption to microbes in activated sludge to the ciprofloxacin selective pressure. This work demonstrates the influence of ciprofloxacin on the activated sludge process, and can provide a useful reference for the assessment of the ecological security of ciprofloxacin.
本研究基于微生物群落和代谢特性，评估了环丙沙星对活性污泥的影响。结果表明，与对照组相比，环丙沙星在 mg/L 水平上抑制了化学需氧量（COD）和氮的代谢，且环丙沙星的浓度略有下降。高通量测序（HTS）结果表明，环丙沙星极大地改变了活性污泥中的微生物群落，特别是硝化螺旋菌门和硝化螺旋菌属。高浓度的环丙沙星刺激了动胶菌的富集，从而降低了活性污泥的稳定性。此外，在这些富集培养物中，气单胞菌中的喹诺酮类耐药蛋白得到了富集，这表明它们在这些富集培养物中具有竞争优势。最后，通过 Tax4Fun 预测了功能谱，揭示了微生物对活性污泥中环丙沙星选择压力的适应。这项工作展示了环丙沙星对活性污泥工艺的影响，可为评估环丙沙星的生态安全性提供有益参考。
