Balentine J D, Greene W B, Bornstein M
Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston.
Lab Invest. 1988 Jan;58(1):93-9.
Fetal mouse spinal cord explants were harvested and allowed to grow in Maximow chambers. Normal appearing matured cultures were subjected to a brief episode of impact trauma by dropping the flat surface of 25 to 105 mg dressmaker's pins from a height of 10 cm directly onto the exposed surface of the culture. Light and electron microscopic studies at selected posttrauma intervals revealed discrete foci of necrosis preceded or accompanied by nerve fiber changes (granular axoplasm, vesicular myelin, pleomorphic spheroids) identical with those documented in spinal cord trauma in vivo. Although no inherent calcification was observed, calcium was added to some of the traumatized cultures and it was subsequently localized by pyroantimonate in the axoplasm; within mitochondria, adjacent to neurofilaments, and in the cytosol. The study indicates that the morphologic sequence of events of spinal cord trauma in vitro are similar to those observed in vivo, and that the latter may occur in the absence of vascular injury.
收集胎鼠脊髓外植体，使其在马克西莫培养室中生长。将外观正常的成熟培养物从10厘米高度直接将25至105毫克女裁缝用大头针的平面掉落至培养物暴露表面，使其遭受短暂的撞击创伤。在选定的创伤后时间间隔进行光镜和电镜研究，发现离散的坏死灶之前或伴有与体内脊髓创伤中记录的相同的神经纤维变化（颗粒状轴浆、泡状髓鞘、多形球体）。虽然未观察到内在钙化，但向一些受创伤的培养物中添加了钙，随后焦锑酸盐将其定位在轴浆中；在线粒体内、神经丝附近和细胞质中。该研究表明，体外脊髓创伤的形态学事件序列与体内观察到的相似，并且后者可能在无血管损伤的情况下发生。
