Feng Peng, Li Zhenqing, Li Yuchen, Zhang Yuelin
Xi'an Medical University, Xi'an, China.
Research Center of Clinical Medicine, Affiliated Hospital of Nantong University, Nantong, China.
Front Mol Biosci. 2020 Dec 10;7:562416. doi: 10.3389/fmolb.2020.562416. eCollection 2020.
The mutation of phosphatase and tensin homolog () genes frequently occur in low-grade gliomas (LGGs) and are deeply associated with a poor prognosis and survival rate. In order to identify the crucial signaling pathways and genes associated with the mutation, we performed bioinformatics analysis on the RNA sequencing results, which were obtained from The Cancer Genome Atlas database. A total of 352 genes were identified as differentially expressed genes (DEGs). The gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis suggested that the DEGs were significantly enriched in categories associated with cell division and multiple metabolic progressions. The histological stage was significantly associated with expression levels. In addition, the mutation was associated with an abundance of cells, neutrophils, macrophages, dendritic cells, and cells during tumor infiltration. The results showed that patients with LGGs harboring the mutation had a poor prognosis and more serious immune cell infiltration occurred depending on the mRNA expression level. These results demonstrated that multiple genes and signaling pathways play a key role in LGG from low grade to high grade, and are associated with mutations. In this study, we outlined an approach to assess the influence of mutations on prognosis, overall survival, and messenger RNA (mRNA) expression. Our results provided alternative strategies for the personalized treatment of patients with LGGs harboring the mutation.
磷酸酶和张力蛋白同源物（）基因的突变频繁发生于低级别胶质瘤（LGGs）中，且与预后不良和生存率密切相关。为了鉴定与该突变相关的关键信号通路和基因，我们对从癌症基因组图谱数据库获得的RNA测序结果进行了生物信息学分析。共鉴定出352个基因作为差异表达基因（DEGs）。基因本体论（GO）和京都基因与基因组百科全书（KEGG）分析表明，这些DEGs在与细胞分裂和多个代谢进程相关的类别中显著富集。组织学阶段与表达水平显著相关。此外，该突变与肿瘤浸润过程中丰富的细胞、中性粒细胞、巨噬细胞、树突状细胞和细胞有关。结果表明，携带该突变的LGGs患者预后不良，且根据mRNA表达水平会发生更严重的免疫细胞浸润。这些结果表明，多个基因和信号通路在LGG从低级到高级的发展过程中起关键作用，并与该突变相关。在本研究中，我们概述了一种评估该突变对预后、总生存期和信使核糖核酸（mRNA）表达影响的方法。我们的结果为携带该突变的LGGs患者的个性化治疗提供了替代策略。
