Purwantini Dattadewi, Santosa R Singgih Sugeng, Santosa Setya Agus, Susanto Agus, Candrasari Dewi Puspita, Ismoyowati Ismoyowati
Faculty of Animal Science, University of Jenderal Soedirman, Indonesia.
Vet World. 2020 Nov;13(11):2301-2311. doi: 10.14202/vetworld.2020.2301-2311. Epub 2020 Nov 3.
Reproductive traits play an important role in population increases and the egg production (EP) abilities of Indonesian local ducks (ILD). The prolactin () gene is a single chain polypeptide hormone belonging to a family of growth hormone genes that are mainly synthesized in the anterior pituitary gland in all vertebrates. It has a significant effect on reproductive traits and EP. Single nucleotide polymorphisms (SNPs) present in are a useful molecular marker for EP. This study aimed to identify the polymorphisms based on these SNPs and to uncover the associations with reproductive traits in ILD.
A total of 280 ILDs consisting of Tegal and Magelang (F0) ducks and their reciprocal crosses, namely, Gallang (F1) and Maggal (F1), were maintained and specific variables were recorded, that is, age at first egg, body weight at first egg, first egg weight, and EP, for 90 days. Allele and genotype frequencies were used to determine the Hardy-Weinberg (H-W) equilibrium. The association between the SNP genotypes of and reproductive traits was analyzed using one-way analysis of variance, following the GLM procedure of SAS. The genotypic effects on the reproductive traits were determined using regression analysis.
This study successfully amplified a polymerase chain reaction product of 190 bp, which was used to identify the SNP. Results indicated that in ILDs is polymorphic. A SNP was found at position 164 nt (c.164G >A), consisting of three different genotypes, namely, GG, GA, and AA. The genotypes of Tegal and Magelang (F0), and Gallang (F1) populations were not in H-W equilibrium. The Maggal population (F1) was in H-W equilibrium. Significant associations were detected between the genotypes and EP in all ILDs (p<0.01), following a regression line of y=2.337x+64.605, with a determination coefficient of 0.0188 (r=0.14).
can be recommended as a candidate gene for reproductive traits in ILD, especially EP.
繁殖性状在种群增长以及印尼本地鸭（ILD）的产蛋能力中起着重要作用。催乳素（PRL）基因是一种单链多肽激素，属于生长激素基因家族，在所有脊椎动物中主要由垂体前叶合成。它对繁殖性状和产蛋量有显著影响。PRL中存在的单核苷酸多态性（SNP）是产蛋量的一种有用分子标记。本研究旨在基于这些SNP鉴定PRL多态性，并揭示其与ILD繁殖性状的关联。
共饲养了280只ILD，包括Tegal和Magelang（F0）鸭及其正反交后代，即Gallang（F1）和Maggal（F1），并记录了特定变量，即开产日龄、开产体重、首枚蛋重和90天产蛋量。使用等位基因和基因型频率来确定哈迪 - 温伯格（H - W）平衡。采用SAS的GLM程序，通过单因素方差分析来分析PRL的SNP基因型与繁殖性状之间的关联。使用回归分析确定基因型对繁殖性状的影响。
本研究成功扩增出190 bp的聚合酶链反应产物，用于鉴定SNP。结果表明，ILD中的PRL具有多态性。在第164 nt处发现一个SNP（c.164G>A），由三种不同基因型组成，即GG、GA和AA。Tegal和Magelang（F0）以及Gallang（F1）群体的基因型不符合H - W平衡。Maggal群体（F1）符合H - W平衡。在所有ILD中，基因型与产蛋量之间检测到显著关联（p<0.01），回归方程为y = 2.337x + 64.605，决定系数为0.0188（r = 0.14）。
PRL可被推荐为ILD繁殖性状尤其是产蛋量的候选基因。
