对紫外线敏感的表皮葡萄球菌突变体的特性研究
Characterization of Staphylococcus epidermidis mutants sensitive to ultraviolet radiation.
作者信息
Guillobel H, Leitão A C
机构信息
Instituto de Biofisica Carlos Chagas Filho, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Brasil.
出版信息
Mutat Res. 1988 Jan;193(1):1-10. doi: 10.1016/0167-8817(88)90002-8.
Five UV-sensitive mutants obtained by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) treatment of the Staphylococcus epidermidis W5 strain were characterized phenotypically by assaying their UV- and MNNG-sensitivities, lysogenic inducibility, host-cell reactivation and Weigle reactivation capacities. The results were compared with those of well-characterized Escherichia coli strains, permitting the identification of: 2 mutants that behave as Uvr- Umu-; 1 mutant that appears analogous to Uvr-; 1 mutant that resembles LexA- and 1 mutant that exhibits a RecA- phenotype. The study of these mutants can contribute to the understanding of the repair mechanisms in S. epidermidis.
通过用N-甲基-N'-硝基-N-亚硝基胍（MNNG）处理表皮葡萄球菌W5菌株获得了五个对紫外线敏感的突变体，通过测定它们对紫外线和MNNG的敏感性、溶原性诱导能力、宿主细胞复活能力和韦格尔复活能力对其进行了表型特征分析。将结果与特征明确的大肠杆菌菌株的结果进行比较，从而鉴定出：2个表现为Uvr-Umu-的突变体；1个类似于Uvr-的突变体；1个类似于LexA-的突变体和1个表现出RecA-表型的突变体。对这些突变体的研究有助于理解表皮葡萄球菌中的修复机制。
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