Petre Alexandru, Balta Cornel, Herman Hildegard, Gharbia Sami, Codreanu Ada, Onita-Mladin Bianca, Anghel-Zurbau Nicoleta, Hermenean Andrei-Gelu, Ignat Simona-Rebeca, Dinescu Sorina, Urzica Iuliana, Drafta Sergiu, Oancea Luminita, Hermenean Anca
Occlusion and Fixed Prosthodontic Department, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania.
"Aurel Ardelean" Institute of Life Sciences, Vasile Goldis Western University of Arad, Romania.
J Adv Res. 2020 Jul 23;28:221-229. doi: 10.1016/j.jare.2020.07.012. eCollection 2021 Feb.
Obtaining a certain bone volume is an important goal in implantology or orthopedics. Thus, after tooth extraction, quite a lot of horizontal and vertical alveolar bone is lost in time and can be detrimental to the implant treatment outcome, while the treatment of critical bone defects is a considerable challenge for surgery.
In this study we designed a new in vivo model as an useful experimental tool to assess guided bone regeneration (GBR) using a computer-aided design/manufacturing (CAD-CAM) space-maintaining barrier.
The barrier was 3D printed with three progressive heights, surgically placed on rat femur, and GBR results were analyzed at 2, 4, and 8 weeks by X-ray and bone mineral density analysis, histology/morphometry and by immunofluorescence and immunohistochemistry for osteogenesis and angiogenesis evaluation.
The obtained results show that the proposed experimental model provides a real-time useful information on progressive bone tissue formation, which depends on the volume of isolated space created for GBR and on molecular events that lead to satisfactory vertical and horizontal bone augmentation and osteointegration.
In conclusion, the proposed customized three-dome space-maintaining barrier is suitable as an experimental tool to assess the potential of using the designed barriers in dentistry and orthopedics to promote the formation of new bone and determine their space- and time-dependent limitations. Meanwhile, guided bone augmentation for dentistry requires subsequent evaluation on an alveolar bone preclinical model followed by clinical implementation.
获得一定的骨量是种植牙学或骨科学中的一个重要目标。因此，拔牙后，大量的水平和垂直牙槽骨会随着时间流失，这可能会对种植治疗结果产生不利影响，而关键骨缺损的治疗对手术来说是一个相当大的挑战。
在本研究中，我们设计了一种新的体内模型，作为一种有用的实验工具，以评估使用计算机辅助设计/制造（CAD-CAM）空间维持屏障的引导骨再生（GBR）。
该屏障采用三种逐渐增加的高度进行3D打印，手术放置在大鼠股骨上，并在2周、4周和8周时通过X射线和骨密度分析、组织学/形态测量以及免疫荧光和免疫组织化学对GBR结果进行分析，以评估成骨和血管生成情况。
获得的结果表明，所提出的实验模型提供了关于渐进性骨组织形成的实时有用信息，这取决于为GBR创建的隔离空间的体积以及导致令人满意的垂直和水平骨增量及骨整合的分子事件。
总之，所提出的定制三穹顶空间维持屏障适合作为一种实验工具，以评估在牙科和骨科学中使用设计的屏障促进新骨形成的潜力，并确定其空间和时间依赖性限制。同时，牙科的引导骨增量需要随后在牙槽骨临床前模型上进行评估，然后进行临床实施。
