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深海贻贝鳃共生的分子分析

Molecular analyses of the gill symbiosis of the bathymodiolin mussel .

作者信息

Wang Hao, Zhang Huan, Zhong Zhaoshan, Sun Yan, Wang Minxiao, Chen Hao, Zhou Li, Cao Lei, Lian Chao, Li Chaolun

机构信息

Center of Deep-Sea Research, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, P. R. China.

Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, P. R. China.

出版信息

iScience. 2020 Dec 7;24(1):101894. doi: 10.1016/j.isci.2020.101894. eCollection 2021 Jan 22.

DOI:10.1016/j.isci.2020.101894
PMID:33364583
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7750550/
Abstract

Although the deep-sea bathymodiolin mussels have been intensively studied as a model of animal-bacteria symbiosis, it remains challenging to assess the host-symbiont interactions due to the complexity of the symbiotic tissue-the gill. Using cold-seep mussel as a model, we isolated the symbiont harboring bacteriocytes and profiled the transcriptomes of the three major parts of the symbiosis-the gill, the bacteriocyte, and the symbiont. This breakdown of the complex symbiotic tissue allowed us to characterize the host-symbiont interactions further. Our data showed that the gill's non-symbiotic parts play crucial roles in maintaining and protecting the symbiosis; the bacteriocytes supply the symbiont with metabolites, control symbiont population, and shelter the symbiont from phage infection; the symbiont dedicates to the methane oxidation and energy production. This study demonstrates that the bathymodiolin symbiosis interacts at the tissue, cellular, and molecular level, maintaining high efficiency and harmonic chemosynthetic micro niche.

摘要

尽管深海贻贝作为动物-细菌共生的模型已得到深入研究，但由于共生组织（鳃）的复杂性，评估宿主与共生体之间的相互作用仍然具有挑战性。以冷泉贻贝为模型，我们分离出了含有细菌细胞的共生体，并对共生关系的三个主要部分——鳃、细菌细胞和共生体进行了转录组分析。这种对复杂共生组织的分解使我们能够进一步表征宿主与共生体之间的相互作用。我们的数据表明，鳃的非共生部分在维持和保护共生关系中起着关键作用；细菌细胞为共生体提供代谢产物，控制共生体数量，并保护共生体免受噬菌体感染；共生体致力于甲烷氧化和能量产生。这项研究表明，深海贻贝共生关系在组织、细胞和分子水平上相互作用，维持着高效和谐的化学合成微生境。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b45/7750550/04be3fbb10ac/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b45/7750550/a9aefb5dac76/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b45/7750550/50310d65a1cb/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b45/7750550/ca5974892587/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b45/7750550/07373c7017ee/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b45/7750550/befef850bf8b/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b45/7750550/14595290d636/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b45/7750550/1a04d0eb26a9/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b45/7750550/96feca985bbb/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b45/7750550/04be3fbb10ac/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b45/7750550/a9aefb5dac76/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b45/7750550/50310d65a1cb/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b45/7750550/ca5974892587/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b45/7750550/07373c7017ee/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b45/7750550/befef850bf8b/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b45/7750550/14595290d636/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b45/7750550/1a04d0eb26a9/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b45/7750550/96feca985bbb/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b45/7750550/04be3fbb10ac/gr8.jpg

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本文引用的文献

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Trends Microbiol. 2020 Oct;28(10):851-866. doi: 10.1016/j.tim.2020.05.016. Epub 2020 Jun 12.
2
Methanotrophic bacterial symbionts fuel dense populations of deep-sea feather duster worms (Sabellida, Annelida) and extend the spatial influence of methane seepage.产甲烷菌共生体为深海羽毛管蠕虫（沙蚕目，环节动物）的密集种群提供燃料，并扩大了甲烷渗漏的空间影响。
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多种共生体之间的代谢交接可能有益于深海贻贝科贻贝。
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Front Microbiol. 2022 Aug 15;13:940766. doi: 10.3389/fmicb.2022.940766. eCollection 2022.
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