胚胎小鼠皮层中 DNA 结合的微染色体维持复合物的成像。

imaging of DNA-bound minichromosome maintenance complex in embryonic mouse cortex.

机构信息

Department of Physiology, School of Medicine, University of Patras, Rio, Patras 26504, Greece.

Advanced Digital Microscopy Facility, Institute for Research in Biomedicine-IRB Barcelona, 08028 Barcelona, Spain.

出版信息

STAR Protoc. 2020 Dec 19;2(1):100234. doi: 10.1016/j.xpro.2020.100234. eCollection 2021 Mar 19.

DOI:10.1016/j.xpro.2020.100234

PMID:33364622

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7753948/

Abstract

The recruitment of the minichromosome maintenance complex (MCM) on DNA replication origins is a critical process for faithful genome duplication termed licensing. Aberrant licensing has been associated with cancer and, recently, with neurodevelopmental diseases. Investigating MCM loading in complicated tissues, such as brain, remains challenging. Here, we describe an optimized approach for the qualitative and quantitative analysis of DNA-bound MCMs in the developing mouse cortex through direct imaging, offering an innovative insight into the research of origin licensing .

摘要

招募微小染色体维持复合物（MCM）在 DNA 复制起点是一个关键的过程，称为许可，以忠实的基因组复制。异常许可与癌症有关，最近也与神经发育疾病有关。在大脑等复杂组织中研究 MCM 加载仍然具有挑战性。在这里，我们描述了一种优化的方法，通过直接成像，对发育中的小鼠皮层中的 DNA 结合的 MCM 进行定性和定量分析，为起源许可的研究提供了新的见解。