Shekhovtsov Sergei V, Shipova Aleksandra A, Poluboyarova Tatiana V, Vasiliev Gennady V, Golovanova Elena V, Geraskina Anna P, Bulakhova Nina A, Szederjesi Tímea, Peltek Sergei E
Department of Molecular Biotechnology, Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
Kurchatov Genomic Center, Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
Front Genet. 2020 Dec 7;11:598196. doi: 10.3389/fgene.2020.598196. eCollection 2020.
(Eisen, 1879) is the only autochthonous Siberian earthworm with a large distribution that ranges from tundra to steppe and broadleaved forests. This species has a very high morphological, ecological, karyological, and genetic diversity, so it was proposed that should be split into several species. However, the phylogeny of the complex was unclear due to the low resolution of the methods used and the high diversity that should have been taken into account. We investigated this question by (1) studying the diversity of the COI gene of throughout its range and (2) sequencing transcriptomes of different genetic lineages to infer its phylogeny. We found that is monophyletic and is split into two clades. The first one includes the pigmented genetic lineages widespread in the northern and western parts of the distribution, and the second one originating from the southern and southeastern part of the species' range and representing both pigmented and non-pigmented forms. We propose to split the complex into two species, and sp. 1 (aff. ), corresponding to these two clades. The currently recognized non-pigmented subspecies will be abolished as a polyphyletic and thus a non-natural taxon, while sp. 1 will be expanded to include several lineages earlier recognized as and .
（艾森，1879年）是唯一一种分布广泛的西伯利亚本土蚯蚓，其分布范围从苔原到草原和阔叶林。该物种具有非常高的形态、生态、核型和遗传多样性，因此有人提议将其划分为几个物种。然而，由于所用方法的分辨率低以及本应考虑的高多样性，该复合体的系统发育尚不清楚。我们通过（1）研究整个分布范围内的COI基因多样性以及（2）对不同遗传谱系的转录组进行测序以推断其系统发育来研究这个问题。我们发现艾森蚯蚓是单系的，并分为两个分支。第一个分支包括分布在北部和西部的有色素遗传谱系，第二个分支起源于该物种分布范围的南部和东南部，包括有色素和无色素两种形态。我们提议将艾森蚯蚓复合体划分为两个物种，即艾森蚯蚓和新种1（近似艾森蚯蚓），分别对应这两个分支。目前公认的无色素亚种将作为一个多系的、因而非自然的分类单元被废除，而新种1将扩大到包括几个早期被认定为艾森蚯蚓和其他种的谱系。
