Niu Mingyang, Jiang Zhen, Xin Xin, Zhu Junling, Yang Jia, Diao Min, Qi Gongjian, Qi Boxiang
Department of Critical Care Medicine, Xuzhou Children's Hospital, Xuzhou Medical University, Xuzhou, Jiangsu 221002, P.R. China.
Exp Ther Med. 2021 Jan;21(1):75. doi: 10.3892/etm.2020.9507. Epub 2020 Nov 25.
Expression of high mobility group protein box 1 (HMGB1) in children with respiratory syncytial virus bronchiolitis and its effect on the inflammatory function of monocytes were investigated. A total of 30 cases of respiratory syncytial viral bronchitis and 30 cases of healthy persons from physical examination were collected from January 2017 to September 2019 in the pediatric department of Xuzhou Children's Hospital, Xuzhou Medical University. HMGB1 expression level in plasma was detected by ELISA. All participants in the study were followed up for 18 months. Human recombinant respiratory syncytial virus (RSV)-A2 virus was used to infect human bronchial epithelial cell line 16HBE, and cell culture supernatant was collected to detect HMGB1. Transwell plate was used to co-culture infected or no-infection groups of epithelial cells and monocytes THP-1. Western blot was used to detect the level of Toll-like receptor (TLR)4 and TLR7 in monocytes. HMGB1 expression level in peripheral blood of children with bronchiolitis was significantly increased compared with that in healthy controls (P<0.0001), and was significantly correlated with the severity of the children's condition (P<0.01). The expression level of HMGB1 was significantly correlated with the number of monocytes, lymphocytes and CRP expression level. HMGB1 was also significantly increased in cell culture supernatant compared with no-infection group (P<0.0001). TLR4 expression in monocytes could be activated by the virus infected cell lines. Follow-up results showed that children with bronchiolitis had a higher incidence of asthma within 18 months (P<0.05). The independent risk factors for children to develop asthma were age, number of monocytes and HMGB1 level. HMGB1 is highly expressed in peripheral blood of children with respiratory syncytial virus bronchitis, and RSV epithelial cells can activate TLR4 expression in monocytes, suggesting that HMGB1 plays an important role in monocyte mediated immune inflammation. HMGB1 expression level is related to the development of asthma in children, which is of great significance for understanding the pathogenesis of bronchiolitis and suggesting the prognosis of children.
研究了高迁移率族蛋白盒1（HMGB1）在呼吸道合胞病毒细支气管炎患儿中的表达及其对单核细胞炎症功能的影响。2017年1月至2019年9月，在徐州医科大学附属徐州儿童医院儿科收集了30例呼吸道合胞病毒性支气管炎患儿和30例体检健康者。采用ELISA法检测血浆中HMGB1表达水平。对研究中的所有参与者进行了18个月的随访。用人重组呼吸道合胞病毒（RSV）-A2病毒感染人支气管上皮细胞系16HBE，收集细胞培养上清液检测HMGB1。用Transwell板将感染或未感染组的上皮细胞与单核细胞THP-1共培养。采用Western blot法检测单核细胞中Toll样受体（TLR）4和TLR7的水平。与健康对照组相比，细支气管炎患儿外周血中HMGB1表达水平显著升高（P<0.0001），且与患儿病情严重程度显著相关（P<0.01）。HMGB1表达水平与单核细胞、淋巴细胞数量及CRP表达水平显著相关。与未感染组相比，细胞培养上清液中HMGB1也显著升高（P<0.0001）。病毒感染的细胞系可激活单核细胞中TLR4的表达。随访结果显示，细支气管炎患儿在18个月内哮喘发病率较高（P<0.05）。儿童发生哮喘 的独立危险因素为年龄、单核细胞数量和HMGB1水平。HMGB1在呼吸道合胞病毒细支气管炎患儿外周血中高表达，RSV上皮细胞可激活单核细胞中TLR4的表达，提示HMGB1在单核细胞介导的免疫炎症中起重要作用。HMGB1表达水平与儿童哮喘的发生有关，这对于理解细支气管炎的发病机制及提示儿童预后具有重要意义。
