Cao Zubing, Gao Di, Xu Tengteng, Tong Xu, Wang Yiqing, Li Yunsheng, Fang Fugui, Ding Jianping, Zhang Xiaorong, Zhang Yunhai
Anhui Provincial Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, , , .
Anim Reprod. 2018 Aug 16;15(1):29-38. doi: 10.21451/1984-3143-AR2017-0027.
Air phase is an indispensable environmental factor affecting oocyte maturation and early embryo development. Human exhaled air was previously proved to be a reliable and inexpensive atmosphere that sustains normal early development of mouse and bovine embryos. However, whether human exhaled air can support maturation (IVM) of porcine oocytes is not yet known. To evaluate the feasibility of maturing oocytes in human exhaled air, we examined oocyte morphology, expression, nuclear and cytoplasmic maturation. We found that cumulus expansion status, expression levels of important for cumulus expansion and the rate of first polar body emission were similar among human exhaled air, 5% O or 20% O in air after IVM of 44 h. Furthermore, the percentage of metaphase II (MII) oocytes showing normal cortical and sub-membranous localization of cortical granules and diffused mitochondrial distribution patterns is comparable among groups. The cleavage, blastocyst rate and total cell number were not apparently different for parthenogenetic activated and somatic cloned embryos derived from MII oocytes matured in three air phases, suggesting oocytes matured in human exhaled air obtain normal developmental competence. Taken together, human exhaled air can efficiently support maturation of porcine oocytes and subsequent early embryonic development.
气相是影响卵母细胞成熟和早期胚胎发育的一个不可或缺的环境因素。先前已证明，人类呼出的空气是一种可靠且廉价的气体环境，能够维持小鼠和牛胚胎的正常早期发育。然而，人类呼出的空气是否能够支持猪卵母细胞的体外成熟（IVM）尚不清楚。为了评估在人类呼出的空气中使卵母细胞成熟的可行性，我们检测了卵母细胞的形态、基因表达、核成熟和胞质成熟情况。我们发现，在44小时体外成熟后，人类呼出的空气、含5%氧气的空气或含20%氧气的空气中卵丘扩展状态、卵丘扩展相关基因的表达水平以及第一极体排放率相似。此外，处于中期II（MII）的卵母细胞中，皮质颗粒呈现正常皮质和亚膜下定位且线粒体呈弥散分布模式的比例在各实验组间相当。由在三种气相环境中成熟的MII卵母细胞孤雌激活和体细胞核移植得到的胚胎，其卵裂率、囊胚率和总细胞数并无明显差异，这表明在人类呼出的空气中成熟的卵母细胞具备正常的发育能力。综上所述，人类呼出的空气能够有效地支持猪卵母细胞的成熟以及随后的早期胚胎发育。
