Liu Wei, He Yuxiao, Ding Jingjing, Chang Qing
Ministry of Ecology and Environment of the People's Republic of China, Nanjing Institute of Environmental Sciences, Jiangsu, People's Republic of China.
Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, Jiangsu, People's Republic of China.
Mitochondrial DNA B Resour. 2019 Jul 12;4(2):2353-2354. doi: 10.1080/23802359.2019.1629349.
The complete mitochondrial DNA genome of the Endangered Spotted Greenshank, , was detected using phenol-chloroform extraction procedure and polymerase chain reaction method. It is a circular molecule of 16,935 bp in size, exhibits the typical structure of mitochondrial genomes of Charadriiformes, containing 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and a control region. Overall base composition of the complete mitochondrial DNA is A (31.7%), T (25.5%), C (29.5%), and G (13.3%), the percentage of A and T (57.2%) is higher than G and C. The phylogenetic relationships of 34 species were reconstructed using the maximum-likelihood and Bayesian inference, and unravel is close to .
采用苯酚 - 氯仿提取法和聚合酶链反应方法，检测了濒危物种小青脚鹬的完整线粒体DNA基因组。它是一个大小为16,935 bp的环状分子，具有鸻形目线粒体基因组的典型结构，包含13个蛋白质编码基因、2个rRNA基因、22个tRNA基因和一个控制区。完整线粒体DNA的总体碱基组成为A（31.7%）、T（25.5%）、C（29.5%）和G（13.3%），A和T的百分比（57.2%）高于G和C。使用最大似然法和贝叶斯推断法重建了34个物种的系统发育关系，结果显示小青脚鹬与[此处原文缺失相关物种信息]关系密切。
