线粒体基因组的特征分析与系统发育分析
Characterization and phylogenetic analysis of mitochondrial genome.
作者信息
Liu Yi, Xu Baohong, Xiao Tiaoyi, Liu Qiaolin
机构信息
Hunan Engineering Technology Research Center of Featured Aquatic Resources Utilization, Hunan Agricultural University, Changsha, PR China.
Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province, Changde, PR China.
出版信息
Mitochondrial DNA B Resour. 2019 Sep 4;4(2):2876-2877. doi: 10.1080/23802359.2019.1660251.
is a kind of famous and precious ornamental fish. In this study, the complete mitochondrial genome yielded by next-generation sequencing of was assembled and analyzed. The total length of the mitochondrial genome is 16,822 bp, with the base composition of 32.40% A, 26.10% T, 26.30% C, and 15.20% G. It contains two ribosomal RNA genes, 13 protein-coding genes, 22 transfer RNA genes, and a major non-coding control region (D-loop region). The arrangement of these genes is the same as that found in the Siluriformes. All the protein initiation codons are ATG, except for cox1 that begins with GTG. The complete mitogenomes of and other 17 species from nine genera were used for phylogenetic analysis by Neighbour-Joining method. The topology demonstrated that all species are divided into three groups (Siluridae, Loricariidae, and Callichthyidae), and the was clustered with and .
是一种名贵的观赏鱼。在本研究中，对 通过下一代测序产生的完整线粒体基因组进行了组装和分析。线粒体基因组全长16,822 bp，碱基组成为32.40%的A、26.10%的T、26.30%的C和15.20%的G。它包含两个核糖体RNA基因、13个蛋白质编码基因、22个转移RNA基因和一个主要的非编码控制区（D-loop区）。这些基因的排列与鲇形目中发现的排列相同。除了cox1以GTG开头外，所有蛋白质起始密码子均为ATG。利用邻接法对 的完整线粒体基因组以及来自九个属的其他17个物种进行了系统发育分析。拓扑结构表明，所有物种分为三组（鲿科、甲鲶科和丽鱼科），并且 与 和 聚类在一起。
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