Do Seong Duk, Rhee Jae-Sung
Department of Marine Science, College of Natural Science, Incheon National University, Incheon 22012, Republic of Korea.
Research Institute of Basic Sciences, Incheon National University, Incheon 22012, Republic of Korea.
Genes (Basel). 2025 Feb 26;16(3):282. doi: 10.3390/genes16030282.
: In this study, we report the complete mitochondrial genome sequence of Weitzman, 1961 (Siluriformes: Callichthyidae), a callichthyid catfish. : DNA sequencing was performed to obtain its complete mitogenome using the HiSeq platform. To assess the phylogenetic relationships, maximum-likelihood and Bayesian inference phylogenetic trees were constructed using two ribosomal RNA (rRNA) genes and all protein-coding sequences (PCGs) concatenated from the mitogenome, along with 31 other Siluriformes mitogenomes. : The complete mitogenome of is 16,579 base pairs in length, with a nucleotide composition of 32.2% A, 26.0% T, 15.3% G, and 26.5% C. It contains 13 PCGs, 22 transfer RNA genes, and 2 rRNA genes. Phylogenetic analysis based on all PCGs and two rRNAs of the complete mitogenome confirms as a sister species of within the subfamily Corydoradinae. In addition, intergenic sequences between and of 21 species of Corydoradinae provide further support for their phylogenetic relationship. : Given the lack of detailed descriptions regarding the length and nucleotide composition of these intergenic sequences, our study contributes valuable insights into the genetic diversity and evolutionary complexity of Callichthyidae.
在本研究中，我们报告了1961年韦茨曼氏兵鲶（Siluriformes: Callichthyidae）的完整线粒体基因组序列，韦茨曼氏兵鲶是一种兵鲶科鲶鱼。使用HiSeq平台进行DNA测序以获得其完整的线粒体基因组。为了评估系统发育关系，利用两个核糖体RNA（rRNA）基因以及从线粒体基因组拼接的所有蛋白质编码序列（PCG），连同其他31种鲶形目线粒体基因组构建了最大似然法和贝叶斯推断系统发育树。韦茨曼氏兵鲶的完整线粒体基因组长度为16,579个碱基对，核苷酸组成为32.2%A、26.0%T、15.3%G和26.5%C。它包含13个PCG、22个转运RNA基因和2个rRNA基因。基于完整线粒体基因组的所有PCG和两个rRNA的系统发育分析证实韦茨曼氏兵鲶是兵鲶亚科内另一种兵鲶的姐妹物种。此外，21种兵鲶亚科物种的韦茨曼氏兵鲶和另一种兵鲶之间的基因间隔序列为它们的系统发育关系提供了进一步支持。鉴于缺乏关于这些基因间隔序列长度和核苷酸组成的详细描述，我们的研究为兵鲶科的遗传多样性和进化复杂性提供了有价值的见解。
